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Methods: Wet Lab
Dr. Jason G. Wallace edited this page Dec 13, 2024
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19 revisions
- General
- Seed Sterilization
- rhAmp Genotyping
- Real Time qPCR
- Whole Bacteria Microbiome Extraction
- Printing Labels
- Plating bacteria
- Opentrons Liquid Handler
The wet lab is a fragile ecosystem that requires precision and care. Before working in the wet lab ensure you have completed all trainings.
For a reminder about sterile technique please see:
Here are the posted Wet Lab Rules:
- SAFETY FIRST
- Know where the first aid, fire blanket, and emergency eye station are.
- No food, drink, or other consumables
- Do not work in the wet lab alone (or at least let someone know you are there)
- Put on gloves!
- When working with dangerous or corrosive reagents wear safety goggles and a lab coat
- Label everything you make with your initials and date
- Put away anything you are not actively using
- Keep your space and the lab organized
- Clean your dishes
- Do not leave frozen or refrigerated reagents out for extended periods of time.
- Please limit crying in the wet lab while others are working
- If faced with a fire in the lab: pause, think, remove it's source of oxygen/fuel, and always report. Water is NOT the answer.
Printing Labels is one of the most challenging tasks you will take upon yourself in the Wallace Lab. Here is a guide to limit the pain.
- Make an Excel table with headers for labels.
- Mailing tab, start mail merge, labels. Brand = Avery, OK
- Select Recipients -> use existing list.
- OK, OK
- Address Block
- Match Fields - First, Middle, Last Name
- OK, Update Labels
- Finish and Merge
- Edit Individual Documents, All, OK
- Center and Enlarge all
- Print -> Print Preferences
- Paper = Manual Print
- Feed Label paper into printer
See this guide in the Journal of Visualized Experiments: Aseptic Laboratory Techniques: Plating Methods. (We especially use the Spread-plating technique with a rod)
See this separate page for the Opentrons robot.