Methods: Wet Lab

Wet Lab Methods

Table of Contents

• General
• Seed Sterilization
• Real Time qPCR
• Whole Bacteria Microbiome Extraction
• Plating bacteria
• Inoculating Fescue Seedlings with Epichloe
• Peanut Nodule Trapping Protocol
• Opentrons Liquid Handler
• rhAmp Genotyping
• Printing Labels

---

---

General

The wet lab is a fragile ecosystem that requires precision and care. Before working in the wet lab ensure you have completed all trainings.

For a reminder about sterile technique please see:

Sterile Technique Guide

Here are the posted Wet Lab Rules:

• SAFETY FIRST
• Know where the first aid, fire blanket, and emergency eye station are.
• No food, drink, or other consumables
• Do not work in the wet lab alone (or at least let someone know you are there)
• Put on gloves!
• When working with dangerous or corrosive reagents wear safety goggles and a lab coat
• Label everything you make with your initials and date
• Put away anything you are not actively using
• Keep your space and the lab organized
• Clean your dishes
• Do not leave frozen or refrigerated reagents out for extended periods of time.
• Please limit crying in the wet lab while others are working
• If faced with a fire in the lab: pause, think, remove it's source of oxygen/fuel, and always report. Water is NOT the answer.

• Printing labels

Microbiology

• Aseptic Laboratory Techniques: Plating Methods. (We especially use the Spread-plating technique with a rod)

Genomics

• rhAmp Genotyping
• Real Time qPCR
• Whole Bacteria Microbiome Extraction
• Running the Opentrons liquid handler

Working with maize seeds

• Seed Sterilization

---