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Move docker image back to openjdk:8u121 so bowtie build works
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Also split bwa tools out into own dockerfile to keep things simple
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ewels committed Aug 30, 2017
1 parent f491c8a commit f5dee4e
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33 changes: 3 additions & 30 deletions Dockerfile
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FROM openjdk:8
# openjdk:8 moved from debian jessie to stretch after u131, which breaks everything (bowtie)
FROM openjdk:8u121

LABEL authors="[email protected],[email protected]" \
description="Docker image containing all requirements for NGI-MethylSeq pipeline"
Expand Down Expand Up @@ -52,12 +53,6 @@ RUN curl -fsSL https://github.com/samtools/samtools/releases/download/1.5/samtoo
make install && \
rm /opt/samtools-1.5.tar.bz2

# Install PicardTools
RUN curl -fsSL https://github.com/broadinstitute/picard/releases/download/2.0.1/picard-tools-2.0.1.zip -o /opt/picard-tools-2.0.1.zip && \
unzip /opt/picard-tools-2.0.1.zip -d /opt/ && \
rm /opt/picard-tools-2.0.1.zip
ENV PICARD_HOME /opt/picard-tools-2.0.1

# Install Bowtie2
RUN mkdir /opt/bowtie2 && \
curl -fsSL https://downloads.sourceforge.net/project/bowtie-bio/bowtie2/2.3.2/bowtie2-2.3.2-source.zip -o /opt/bowtie2/bowtie2.zip && \
Expand All @@ -82,27 +77,5 @@ RUN mkdir /opt/Qualimap && \
ln -s /opt/Qualimap/qualimap_v2.2.1/qualimap /usr/local/bin/qualimap && \
rm /opt/Qualimap/qualimap.zip

# Install BWA
RUN mkdir /opt/bwa && \
curl -fsSL https://downloads.sourceforge.net/project/bio-bwa/bwa-0.7.15.tar.bz2 -o /opt/bwa/bwa.tar.bz2 && \
tar xvjf /opt/bwa/bwa.tar.bz2 -C /opt/bwa/ && \
cd /opt/bwa/bwa-0.7.15/ && \
make && \
ln -s /opt/bwa/bwa-0.7.15/bwa /usr/local/bin/bwa && \
rm /opt/bwa/bwa.tar.bz2

# Install bwa-meth
RUN pip install toolshed && \
pip install git+git://github.com/brentp/bwa-meth.git

# Install MethylDackel
RUN mkdir /opt/MethylDackel && \
curl -fsSL https://github.com/dpryan79/MethylDackel/archive/0.2.1.zip -o /opt/MethylDackel/MethylDackel.zip && \
unzip /opt/MethylDackel/MethylDackel.zip -d /opt/MethylDackel && \
cd /opt/MethylDackel/MethylDackel-0.2.1 && \
make && \
make install prefix=/usr/local/bin && \
rm /opt/MethylDackel/MethylDackel.zip

# Install MultiQC
RUN pip install multiqc
RUN pip install git+https://github.com/ewels/MultiQC.git
91 changes: 91 additions & 0 deletions Dockerfile-bwa
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@@ -0,0 +1,91 @@
FROM openjdk:8

LABEL authors="[email protected],[email protected]" \
description="Docker image containing all requirements for NGI-MethylSeq pipeline"

# Install container-wide requrements gcc, pip, zlib, libssl, make, libncurses, fortran77, g++, R
RUN apt-get update && \
apt-get install -y --no-install-recommends \
g++ \
gcc \
gfortran \
libbz2-dev \
libcurl4-openssl-dev \
libgsl0-dev \
liblzma-dev \
libncurses5-dev \
libpcre3-dev \
libreadline-dev \
libssl-dev \
libtbb-dev \
make \
python-dev \
zlib1g-dev \
&& rm -rf /var/lib/apt/lists/*

# Install pip
RUN curl -fsSL https://bootstrap.pypa.io/get-pip.py -o /opt/get-pip.py && \
python /opt/get-pip.py && \
rm /opt/get-pip.py

RUN curl -fsSL http://www.bioinformatics.babraham.ac.uk/projects/fastqc/fastqc_v0.11.5.zip -o /opt/fastqc_v0.11.5.zip && \
unzip /opt/fastqc_v0.11.5.zip -d /opt/ && \
chmod 755 /opt/FastQC/fastqc && \
ln -s /opt/FastQC/fastqc /usr/local/bin/fastqc && \
rm /opt/fastqc_v0.11.5.zip

# Install cutadapt
RUN pip install cutadapt

# Install TrimGalore
RUN mkdir /opt/TrimGalore && \
curl -fsSL https://github.com/FelixKrueger/TrimGalore/archive/0.4.4.zip -o /opt/TrimGalore/0.4.4.zip && \
unzip /opt/TrimGalore/0.4.4.zip -d /opt/TrimGalore && \
ln -s /opt/TrimGalore/TrimGalore-0.4.4/trim_galore /usr/local/bin/trim_galore && \
rm /opt/TrimGalore/0.4.4.zip

# Install SAMTools
RUN curl -fsSL https://github.com/samtools/samtools/releases/download/1.5/samtools-1.5.tar.bz2 -o /opt/samtools-1.5.tar.bz2 && \
tar xvjf /opt/samtools-1.5.tar.bz2 -C /opt/ && \
cd /opt/samtools-1.5 && \
make && \
make install && \
rm /opt/samtools-1.5.tar.bz2

# Install PicardTools
RUN curl -fsSL https://github.com/broadinstitute/picard/releases/download/2.0.1/picard-tools-2.0.1.zip -o /opt/picard-tools-2.0.1.zip && \
unzip /opt/picard-tools-2.0.1.zip -d /opt/ && \
rm /opt/picard-tools-2.0.1.zip
ENV PICARD_HOME /opt/picard-tools-2.0.1

# Install Qualimap
RUN mkdir /opt/Qualimap && \
curl -fsSL https://bitbucket.org/kokonech/qualimap/downloads/qualimap_v2.2.1.zip -o /opt/Qualimap/qualimap.zip && \
unzip /opt/Qualimap/qualimap.zip -d /opt/Qualimap && \
ln -s /opt/Qualimap/qualimap_v2.2.1/qualimap /usr/local/bin/qualimap && \
rm /opt/Qualimap/qualimap.zip

# Install BWA
RUN mkdir /opt/bwa && \
curl -fsSL https://downloads.sourceforge.net/project/bio-bwa/bwa-0.7.15.tar.bz2 -o /opt/bwa/bwa.tar.bz2 && \
tar xvjf /opt/bwa/bwa.tar.bz2 -C /opt/bwa/ && \
cd /opt/bwa/bwa-0.7.15/ && \
make && \
ln -s /opt/bwa/bwa-0.7.15/bwa /usr/local/bin/bwa && \
rm /opt/bwa/bwa.tar.bz2

# Install bwa-meth
RUN pip install toolshed && \
pip install git+git://github.com/brentp/bwa-meth.git

# Install MethylDackel
RUN mkdir /opt/MethylDackel && \
curl -fsSL https://github.com/dpryan79/MethylDackel/archive/0.2.1.zip -o /opt/MethylDackel/MethylDackel.zip && \
unzip /opt/MethylDackel/MethylDackel.zip -d /opt/MethylDackel && \
cd /opt/MethylDackel/MethylDackel-0.2.1 && \
make && \
make install prefix=/usr/local/bin && \
rm /opt/MethylDackel/MethylDackel.zip

# Install MultiQC
RUN pip install multiqc

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