Camera

Description:

Our system makes use of a camera attached to a 4mm lens and an infrared filter. Each camera connects to a local computer via USB3 and produces an .mp4 video file every 4 hours. We use Bandicam to record/output at a scaled resolution of 640x512 at 30 fps.

The Digital Homecage system has a perpetually lit infrared(IR) LED backlight behind a translucent acrylic plexiglass panel (1/4” x 13.5” x 24”), which diffuses the IR light, allowing for even illumination. The wavelength of the infrared light falls below the detection threshold for mice, allowing for continuous recording, even in dark hours.

We will use HomeCageScan (a commercial rodent behavioral analysis software), which uses .mp4 files, to analyze our recorded videos.

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Components:

1. Camera: CABR133, 1280 x 1024 1/2" Monochrome CMOS USB3 Camera Basler Ace U acA1300-200um; Camera Software/Camera Settings
2. Lens: LSBR200, 1/2.5" 4 mm 5 MegaPixel Lens, Basler C125-0418-5M
3. IR filter: FRMS208, NIR Bandpass Filter, 46.0 mm Thread Midwest Optical Filters
4. Lens adapter for IR filter: LSBR903, Filter Adapter for Basler Lens
5. Data and power cable for camera: CABR133
6. Camera stand: 3D printed. URL: TBD

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Diagrams(for setting up EPHYS syncing):

Images needed

3-D Print files for camera tripod:

Software/Drivers needed:

1) DOWNLOAD BASLER PYLON FIRST:

a) Download Basler Pylon v.6.2 from here: https://www.baslerweb.com/en/downloads/software-downloads/software-pylon-6-2-0-windows/

b) Download Basler Pylon runtime from here: https://www.baslerweb.com/en/downloads/software-downloads/runtime-pylon-6-2-0-windows/

For video recording WITHOUT electrophysiology:

Purchase the full version of Bandicam video recording software (~$45; 1 license per camera): https://www.bandicam.com/

For video recording WITH electrophysiology:

Purchase Streampix8 (~$1495; 4 (or maybe even more) cameras per license/computer.)