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Updated README
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wyang17 authored May 16, 2018
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## Pipeline ##

#### Inputs ####
The pipeline requires the following inputs:
- BEDfile of Transposable Elements of interest
- STAR index of genome build of interest

If you have trouble obtaining these items on your own, you can use the steps fetch_ to download the chromosome fasta files, Repeatmasker file and create a STAR index with your transposable elements of interest.




#### Pipeline Steps ####

<img align="center" width="825" height="600" src="images/overview_squire.png">

Preparation
Preparation Stage
1) [Fetch:](#squire-fetch)
Downloads input files from RefGene and generates STAR index
Only needs to be done once initially to acquire genomic input files or if a new build is desired.

2) [Clean:](#squire-clean)
Filters Repeatmasker file for Repeats of interest, collapses overlapping repeats, and returns as BED file.

Quantification
Quantification Stage

1) [Map:](#squire-map)
Aligns RNAseq data
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Quantifies RNAseq reads aligning to TEs


Analysis
Analysis Stage

1) [Call:](#squire-call)
Compiles and outputs differential expression from multiple alignments

Follow-up
Follow-up Stage
1) [Draw:](#squire-draw)
Creates BEDgraphs from RNAseq data

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* `qsub -cwd call.sh arguments.sh`
* `qsub -cwd loop_draw.sh arguments.sh`

5. If a memory or segmentation fault error occurs, edit the `#$ -l mem_free` and `#$ -l h_vmem` lines to increase memory usage for the appropriate script.
5. If a memory or segmentation fault error occurs, edit the `#$ -l mem_free` and `#$ -l h_vmem` lines to increase memory usage for the appropriate script.

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