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v.1.4.2.
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tomas-fer authored May 31, 2017
1 parent b21c277 commit 4c4c643
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Showing 19 changed files with 23 additions and 23 deletions.
2 changes: 1 addition & 1 deletion HybPhyloMaker0a_preparedata.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 0a - Download & prepare data *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker0b_preparereference.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 0b - Prepare pseudoreference *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker0c_Rsetup_MetaCentrum.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 0c - Setup R packages on Metacentrum *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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8 changes: 4 additions & 4 deletions HybPhyloMaker1_rawprocess.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 01 - Raw data processing *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# * based on Weitemier et al. (2014), Applications in Plant Science 2(9): 1400042*
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#Adapter and general quality trimming
echo "Adapter removal & quality trimming..."
if [[ $location == "1" ]]; then
java -jar /software/trimmomatic-0.32/dist/jar/trimmomatic-0.32.jar PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../NEBNext-PE.fa:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
java -jar /software/trimmomatic-0.32/dist/jar/trimmomatic-0.32.jar PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../${adapterfile}:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
#trimmomatic PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../NEBNext-PE.fa:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
elif [[ $location == "2" ]]; then
java -d64 -server -XX:MaxHeapSize=10g -jar ../trimmomatic-0.33.jar PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../NEBNext-PE.fa:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
java -d64 -server -XX:MaxHeapSize=10g -jar ../trimmomatic-0.33.jar PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../${adapterfile}:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
else
java -jar ../trimmomatic-0.33.jar PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../NEBNext-PE.fa:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
java -jar ../trimmomatic-0.33.jar PE -phred33 $file-noPhiX_1.fq.gz $file-noPhiX_2.fq.gz $file-1P $file-1U $file-2P $file-2U ILLUMINACLIP:../${adapterfile}:2:30:10 LEADING:20 TRAILING:20 SLIDINGWINDOW:5:20 MINLEN:36 >Trimmomatic.log 2>&1
fi
#Copy trimmed reads to home
if [[ $location == "1" ]]; then
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2 changes: 1 addition & 1 deletion HybPhyloMaker3_generatepslx.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 03 - Process consensus after mapping, make pslx files *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# * based on Weitemier et al. (2014), Applications in Plant Science 2(9): 1400042*
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2 changes: 1 addition & 1 deletion HybPhyloMaker4_processpslx.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 04 - Process pslx files *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# * based on Weitemier et al. (2014), Applications in Plant Science 2(9): 1400042*
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2 changes: 1 addition & 1 deletion HybPhyloMaker4b_correctframe_translate.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 04b - Put exons to correct reading frame and translate *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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4 changes: 2 additions & 2 deletions HybPhyloMaker5_missingdataremoval.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 05 - Missing data handling *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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#Calculate percentage of missing data as integer (for evaluation in bash)
percentage=$(echo -e "scale=0;($count * 100) / $y" | bc)
echo -e "$header\t$percentageprint" >> ${file}_${MISSINGPERCENT}percN.fas
if [ "$percentage" -lt "$MISSINGPERCENT" ]; then
if [ "$percentage" -le "$MISSINGPERCENT" ]; then
echo "$line" >> ${file}_modif${MISSINGPERCENT}.fas
else
echo -e "Deleting $header with $percentageprint of missing data"
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2 changes: 1 addition & 1 deletion HybPhyloMaker6a2_RAxML_trees_summary.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 06a2 - summary of RAxML gene trees *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker6a_RAxML_for_selected.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 06a - RAxML gene tree building *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker6b_FastTree_for_selected.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 06b - FastTree gene tree building *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker7_roottrees.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 07 - Root gene trees *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker8a_astral.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 08a - Astral species tree *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker8b_astrid.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 08b - Astrid species tree *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker8c_mrl.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 08c - MRL species tree *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker8e_concatenatedFastTree.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 08e - concatenated species tree *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker8f_concatenatedExaML.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 08f - ExaML concatenated tree *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion HybPhyloMaker9_update_trees.sh
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# ********************************************************************************
# * HybPhyloMaker - Pipeline for Hyb-Seq data processing and tree building *
# * Script 09 - Update trees *
# * v.1.4.1 *
# * v.1.4.2 *
# * Tomas Fer, Dept. of Botany, Charles University, Prague, Czech Republic, 2017 *
# * [email protected] *
# ********************************************************************************
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2 changes: 1 addition & 1 deletion install_software.sh
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# Tomas Fer, 2017 #
# [email protected] #
# https://github.com/tomas-fer/HybPhyloMaker #
# v.1.4.0 #
# v.1.4.2 #
##########################################################################################################################

#Carefully set your distribution
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