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Sequence generation issue via CLI tool #1

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penuts7644 opened this issue Dec 9, 2018 · 0 comments
Open

Sequence generation issue via CLI tool #1

penuts7644 opened this issue Dec 9, 2018 · 0 comments

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@penuts7644
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Hi, I have an issue when running the olga-generate_sequences command via terminal. Im using the following exact command: olga-generate_sequences -d ',' --VDJ_model_folder olga_input -o cdr3_seqs.csv -n 50.

My input anchor csv files are comma separated and all the model files including V and J gene anchor files are located in the olga_input directory.

The program seems to run (it shows the starting sequence generation text in the terminal). Though it never generates a sequence and thus the output file remains empty (even after over 30 minutes of run time).

When pressing ctrl-c I get the following:

^CTraceback (most recent call last):
  File "/Users/woutvanhelvoirt/Documents/Virtualenvs/python2.7.15_1/bin/olga-generate_sequences", line 11, in <module>
    sys.exit(main())
  File "/Users/woutvanhelvoirt/Documents/Virtualenvs/python2.7.15_1/lib/python2.7/site-packages/olga/generate_sequences.py", line 286, in main
    ntseq, aaseq, V_in, J_in = seq_gen.gen_rnd_prod_CDR3(conserved_J_residues)
  File "/Users/woutvanhelvoirt/Documents/Virtualenvs/python2.7.15_1/lib/python2.7/site-packages/olga/sequence_generation.py", line 211, in gen_rnd_prod_CDR3
    recomb_events = self.choose_random_recomb_events()
  File "/Users/woutvanhelvoirt/Documents/Virtualenvs/python2.7.15_1/lib/python2.7/site-packages/olga/sequence_generation.py", line 277, in choose_random_recomb_events
    recomb_events['delDl'] = delDldelDr_choice/self.num_delDr_poss
KeyboardInterrupt

Im using Python version 2.7.15

Cheers, Wout

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