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Error in cbind count matrices #12

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mvochteloo opened this issue Dec 6, 2023 · 0 comments
Open

Error in cbind count matrices #12

mvochteloo opened this issue Dec 6, 2023 · 0 comments

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@mvochteloo
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On line 83 of Singlet_QC_Figures.R the count matrices are combined using
counts <- do.call(cbind, counts_list)

Since CellRanger v6.0.0 it is default behaviour to only output barcodes with at least one read, rather than all barcodes in the whitelist source. As a result, combining raw_feature_bc_matrix files from pools generated using this or newer versions of CellRanger results in the following error occurs:
Error in cbind.Matrix(x, y, deparse.level = 0L) : number of rows of matrices must match

Merging pools as Seurat objects should resolve this issue (Seurat v4 or Seurat >=v5.0.1)

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