The Disease Resistance Analysis and Gene Orthology (DRAGO 2) is the second version of a pipeline to annotate resistance genes. DRAGO 2 can be launched on any transcriptome or proteome, thus the input can be either DNA or protein sequences in FASTA format. It detects LRR, Kinase, NBS and TIR domains from 60 HMM modules created for this purpose using HMMER v3 package and it is also able to detect CC and TM domains using COILS 2.2 and TMHMM 2.0c programs. As output, DRAGO 2 gives a table with the class of the query sequence and each domain found with the corresponding coordinates.
DRAGO2 is available in PRGdb (http://prgdb.org). PRGdb is an open and updated space about Pathogen Receptor Genes (PRGs), in which all information available about these genes is stored, curated and discussed. The purpose of our work is creating a worldwide community working on plant resistance genes with a constant update on all aspects of this research field and to encourage scientists to be actors of the discussion and of the data exchange.
When it is desired to analyze thousands of sequences at a time (e.g. full transcriptome or proteome), the analysis through the web interface might not be suitable. That is why we created an API to perform DRAGO2 analyses in a programmatic way. How to call the script?
bash drago2api.sh sequences.fasta > output.txt
It outputs a tab-separated table similar to the one given by the web interface. If you use this tool, please cite us:
Osuna-Cruz CM, Paytuvi-Gallart A, Di Donato A, Sundesha V, Andolfo G, Aiese Cigliano R, Sanseverino W, Ercolano MR.
PRGdb 3.0: a comprehensive platform for prediction and analysis of plant disease resistance genes.
Nucleic Acids Research 2017. doi: 10.1093/nar/gkx1119