🌱 I’m currently working as a Graduate Research Assistant at Ohio University, and concurrently

🌱 I'm Pursuing Masters in Biological Science at Ohio University, Ohio.

👯 I’m looking to collaborate on projects related to Next-Generation-Sequencing Data Analysis

💬 I love discussing Biology, Biotechnology and Data Analysis

Graduate Research Assistant

• Leading a predator-diet analysis project for Department of Natural Resources as part of the master’s thesis, leveraging DNA metabarcoding to unravel dietary patterns of North American predators in diverse ecosystem.
• Developing an innovative bioinformatics tool to streamline diet-based Next-Generation Sequencing metagenomic studies, enhancing efficiency in prey identification from predator’s fecal samples through optimized genomic approaches.

Graduate Teaching Assistant

• Led the undergraduate course “Biological Sciences Lab I (BIOS 1705)”, guiding over 40 students for hands-on biological experiments.
• Assisted in the designing and grading of exams, lab reports, and assignments, ensuring timely feedback and comprehensive assessment of student performance.

Junior Research Intern

• Participated in laboratory work for ongoing PhD project on Antibiotic Sensitivity Testing of medications commonly used for treating mastitis in cattle in Nepal.
• Involved in conducting DNA extraction and PCR from fecal samples for the diet study of Snow Leopards in Nepal.

Junior Research Intern

• Conducted a native pollinator survey as a part of undergraduate thesis to document pollinator species among residents in Rukum West, Nepal, with aim to facilitate better agricultural practices and conservation efforts.
• Fostered technology adoption among local farmers in the hilly regions of Nepal by training them on mobile applications designed for agricultural use.

• 👩‍💻 Programming: Python, R, Bash, Git, High Performance Computing, Slurm
• 📚 Laboratory: DNA extraction, PCR, Gel Electrophoresis, Cell Culture, Western Blotting, SDS Page
• 🗄️ Bioinformatics Tools : Next-Generation Sequencing Data Analysis, Data Mining, Pipeline Creation, etc

✔️ Masters in Biological Science Ohio University, USA Anticipated Graduation: May 2025 Major: Ecology and Evolution

✔️ Bachelor of Science in Agriculture Agriculture & Forestry University, Nepal 2018-2022

Metagenomic Analysis of Environmental Samples

• Developed and published a Bash pipeline with Conda environment management for Illumina data, performing QC, trimming, assembly, taxonomic assignment, and visualization for metagenomic studies.

Whole Exome Sequencing of a Family Trio

• Conducted variant calling and annotation on exome data from a family trio using HPC with SLURM for large-scale genomic data processing.

RNA-seq Analysis of Rat Samples[Ongoing]

• Reproduced scRNA-seq analysis pipeline using Snakemake, performing quality control, dimensionality reduction, and differential expression analysis on rat sample data.

Ecological Model Simulator

• Built a web application with python that explains and simulates a mathematical model of interspecific competition based on the input parameters set by the user

• 7th GEDGWGG eDNA Workshop @ Columbus, OH Sep 2024
• 103rd Annual Meeting of the American Society of Mammologists @ Boulder, CO Jun 2024
• Ohio University Student Expo @ Athens, OH Apr 2024