WADE provides a flexible and customizable method to extract specific genes from a large number of genomes at once.
Using BLAST to interrogate assembled genomes, current molecular analyses include antimicrobial resistance, toxins, virulence profiles and several multi-locus sequence typing (MLST) schemes. The Virulence Factor DataBase (VFDB) and antimicrobial resistance factor databases CARD, ARG-ANNOT and ResFinder have also been made available.
Tabular results are output in a format that is compatible for LabWare uploads. These results can consist of simple “Positive-Negative” results corresponding to presence or absence of a queried gene. Curated multi-fasta lookup files can be provided for molecular determinants to create molecular profiles of affective mutations. Fasta file outputs of gene sequences extracted from the genomes can readily be loaded into sequence aligners to correlate nucleotide differences to phenotypic observations.
Copyright Government of Canada 2018
Written by: National Microbiology Laboratory, Public Health Agency of Canada
Licensed under the Apache License, Version 2.0 (the "License"); you may not use this work except in compliance with the License. You may obtain a copy of the License at:
http://www.apache.org/licenses/LICENSE-2.0
Unless required by applicable law or agreed to in writing, software distributed under the License is distributed on an "AS IS" BASIS, WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied. See the License for the specific language governing permissions and limitations under the License.
Gary van Domselaar: [email protected]