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title: TEST | ||
element: lab | ||
layout: default | ||
--- | ||
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## Objectives | ||
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- To learn how to download data from the SRA | ||
- To learn how to filter a fastq file | ||
- To understand the structure and format of genome files | ||
- To learn tricks for manipulating a fastq file | ||
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### | ||
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Today we're going to learn how to access sequence data and prepare it for analysis. Please login to | ||
the "indri" server and go to your home directory to start. | ||
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As a first step, we need to download some sequence data. The North American repository of sequence data | ||
is the Sequence Read Archive (SRA). Whenever a researcher wants to publish a genomic | ||
analysis, they need to upload their raw data to the SRA so that other | ||
researchers can use replicate their results or use it for other purposes. | ||
The sample we're going to work with is identified as DRR053219. | ||
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### Activity | ||
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- What species is this sample from? | ||
- What project was this sample sequenced for? | ||
- Each data file in the SRA has multiple layers of metadata. Specifically Bioproject, Biosample, Experiment and Run. | ||
Explain the difference between each of these layers. | ||
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### | ||
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```bash | ||
module load StdEnv/2023 gcc/12.3 sra-toolkit/3.0.9 | ||
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