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mag 2.3.0 - Red Cow

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@jfy133 jfy133 released this 02 Mar 10:12
· 1069 commits to master since this release
c9468cb

Added

  • #350 - Adds support for CheckM as alternative bin completeness and QC tool (added by @jfy133 and @skrakau)
  • #353 - Added the busco_clean parameter to optionally clean each BUSCO directory after a successful (by @prototaxites)
  • #361 - Added the skip_clipping parameter to skip read preprocessing with fastp or adapterremoval. Running the pipeline with skip_clipping, keep_phix and without specifying a host genome or fasta file skips the FASTQC_TRIMMED process (by @prototaxites)
  • #365 - Added CONCOCT as an additional (optional) binning tool (by @jfy133)
  • #366 - Added CAT_SUMMARISE process and cat_official_taxonomy parameter (by @prototaxites)
  • #372 - Allow CAT_DB to take an extracted database as well as a tar.gz file (by @prototaxites).
  • #380 - Added support for saving processed reads (clipped, host removed etc.) to results directory (by @jfy133)
  • #394 - Added GUNC for additional chimeric bin/contamination QC (added by @jfy133)

Changed

  • #340,#368,#373 - Update to nf-core 2.7.2 TEMPLATE (by @jfy133, @d4straub, @skrakau)
  • #373 - Removed parameter --enable_conda. Updated local modules to new conda syntax and updated nf-core modules (by @skrakau)
  • #385 - CAT also now runs on unbinned contigs as well as binned contigs (added by @jfy133)
  • #399 - Removed undocumented BUSCO_PLOT process (previously generated *.busco_figure.png plots unsuitable for metagenomics) (by @skrakau).

Fixed

  • #345 - Bowtie2 mode changed to global alignment for ancient DNA mode (--very-sensitive mode) to prevent soft clipping at the end of reads when running in local mode. (by @maxibor)
  • #349 - Add a warning that pipeline will reset minimum contig size to 1500 specifically MetaBAT2 process, if a user supplies below this threshold. (by @jfy133)
  • #352 - Escape the case in the BUSCO module that BUSCO can just detect a root lineage but is not able to find any marker genes (by @alexhbnr)
  • #355 - Include error code 21 for retrying with higher memory for SPAdes and hybridSPAdes (by @mglubber)

Dependencies

Tool Previous version New version
BUSCO 5.1.0 5.4.3
BCFtools 1.14 1.16
Freebayes 1.3.5 1.3.6
SAMtools 1.15 1.16.1