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additionalMods reduction, improved predicted mods discovery and isodecoder name shortening

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@drewjbeh drewjbeh released this 08 Jun 13:26
· 371 commits to master since this release

Updates

  • Reduced additional mods file to contain only inosine 34. This allows mim-tRNAseq to discover and annotate other mods on its own and reduces misalignment, e.g. human Asp-GTC-3
  • New mods discovered after round 1 of alignment are then subtracted from predictedMods so that their validity can be rechecked after alignment improvements in round 2. Only these are then added to predictedMods
  • Isodecoder names now exclude 2nd number in name (except "chr" containing names) in all outputs and plots
  • Stops and misinc heatmaps no longer plot gap information ("-")
  • knownMods.csv renamed to allMods.csv as it includes Modomics, additionalMods, and predictedMods sites

Bugfixes

  • allMods.csv positions now 1-based
  • --misinc-thresh now used for heatmap row annotations (i.e. stop and misinc site counts)
  • Duplicates in countTable no longer removes as pandas issue removes non-duplicated rows