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Parsnp v2.0.0

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@bkille bkille released this 05 Jan 17:01
· 80 commits to master since this release

What's Changed

  • You can now pass a newline-separated file of paths to query sequences to -d in addition to directories and command-line lists.
  • Adds --partition flag, which splits recruited genomes into partitions of size --partition-size (default 50).
  • Adds --no-recruit option which skips the recruitment step, but still drops genomes if their size differs substantially from the reference.
  • Fixes multiple bugs in the output:
    • Output can now be parsed by BioPython's AlignIO module with the "mauve" format
    • LCBs no longer overlap
    • Ambiguous base pairs and small contigs no longer lead to incorrect coordinates
    • VCF now contains the correct reference allele.
  • FastANI now guarantees at least 100 segments (unless it requires a fragment length < 500)
  • Adds --min-ref-cov option (default 90), which when used with --use-ani, removes query genomes that do not cover at least 90% of the reference.
  • Output folder has been reorganized to separate logs and config files from the main output.