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ISS decoding module #448

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rukhovich
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NB! Unready yet

This was implemented on MCMicro hackathon 2022 Heidelberg

This is a new module for in situ sequencing pipeline that in our implementation uses starfish for decoding

Input for the pipeline is one input folder that should contain:

  • codebook
  • stitched, registered input images

The workflow is placed after registration

The workflow contains 3 processes:

  • tile_images: tiles images to small pieces that can be proceeded by starfish (or any other decoding software) separately
  • starfish_convert: converts codebook and tiles to json file compatible with starfish
  • starfish_decode: runs starfish decoding

@jmuhlich jmuhlich marked this pull request as draft November 1, 2022 23:57
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jmuhlich commented Nov 1, 2022

Hi! Thanks for this contribution. I converted the PR to a Draft to indicate more clearly that it's not ready, as you say.

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