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lpantano committed Jun 24, 2024
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Expand Up @@ -8,6 +8,17 @@ We are developing platforms for each analysis type we have experience with at HC

Go to each analysis type section to learn how to use our platform.

## Set up package

* Using O2 open OnDemand:
* Remove `bcbio` from you `PATH` by commenting the line in your `.bashrc` if you have it
* Start Rstudio With these modules on: `gcc/9.2.0 imageMagick/7.1.0 geos/3.10.2 cmake/3.22.2 R/4.3.1 fftw/3.3.10 gdal/3.1.4 udunits/2.2.28 boost/1.75.0`
* When the session is started, set you library path typing this command in your console Rstudio window: `.libPaths("/n/app/bcbio/R4.3.1")`

* load bcbioR with `library(bcbioR)`
* Make sure the version is 0.1.3 with this command `packageVersion("bcbioR")` or re-install with `devtools::install_github("bcbio/bcbioR",build_manual = TRUE, build_vignettes = TRUE)`, if you are in your own environment


## General Project

This set up needs `bcbioR` and `usethis` packages.
Expand All @@ -23,13 +34,6 @@ Make sure the version is 0.1.3 with this command `packageVersion("bcbioR")` or r

## RNAseq

* Using O2 open OnDemand:
* Remove `bcbio` from you `PATH` by commenting the line in your `.bashrc` if you have it
* Start Rstudio With these modules on: `gcc/9.2.0 imageMagick/7.1.0 geos/3.10.2 cmake/3.22.2 R/4.3.1 fftw/3.3.10 gdal/3.1.4 udunits/2.2.28 boost/1.75.0`
* When the session is started, set you library path typing this command in your console Rstudio window: `.libPaths("/n/app/bcbio/R4.3.1")`

* load bcbioR with `library(bcbioR)`
* Make sure the version is 0.1.3 with this command `packageVersion("bcbioR")` or re-install with `devtools::install_github("bcbio/bcbioR",build_manual = TRUE, build_vignettes = TRUE)`, if you are in your own environment
* Follow this instruction https://bcbio.github.io/bcbioR/#set-base-project to start you project
* Follow this instruction https://bcbio.github.io/bcbioR/#set-rnaseq-report-folder to start your RNAseq analysis

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