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Experimental procedures followed established techniques using the Chromium Single Cell 5’ Library & Gel Bead Kit (10x Genomics; https://assets.ctfassets.net/an68im79xiti/6v4HtRCmFwamyVW4rnx1tV/90a8d2e6b411a3779616edf17a984ab0/CG000086_ChromiumSingleCellV_D_J_ReagentKits_UG_RevL.pdf). Briefly, magnetically enriched CD8+ T cells in single-cell suspension were loaded onto a 10X Genomics Chip A and emulsified with 5’ Single Cell GEM beads using a Chromium™ Controller (10x Genomics). TCR libraries were constructed from the barcoded cDNAs using the 10x Genomics Chromium™ Single Cell V(D)J Enrichment Kit, Mouse T Cell (Translational Science Laboratory at the Medical University of South Carolina). Sequencing was performed on each sample (approximately 50 million reads/sample) using a NovaSeq S4 flow cell (Illumina) at the VANTAGE facility (Vanderbilt University Medical Center).
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armish
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Write down the methods
Collect and share detailed protocols
Oct 28, 2019
This is from Marty on our 10X protocol:
The text was updated successfully, but these errors were encountered: