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Explore an alternative way to clone TILs by first single-cell TCR-seq'ing them and then transforming non-exhausted T cells with these TIL TCRs

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TCR transfer

Explore an alternative way to clone TILs by first single-cell TCR-seq'ing them and then transforming non-exhausted T cells with these TIL TCRs

Part I - Extract TCRs from TILs

TCR transfer part I

Part II - Test TCRs against cell lines

TCR transfer part II

Materials

Plasmids

ID Plasmid Gene/Insert Vector Type
131033 pcDNA3.1(+)-OTI-TCRA_p2_TCRB TCRA (Mus musculus), TCRB (Mus musculus) Mammalian Expression
131034 pcDNA3.1(+)-OTI-TCRB_p2_TCRA TCRB (Mus musculus), TCRA (Mus musculus) Mammalian Expression
131035 pcDNA3.1(+)-OTI-TCRA TCRA (Mus musculus) Mammalian Expression
131036 pcDNA3.1(+)-OTI-TCRB TCRB (Mus musculus) Mammalian Expression
138983 pcDNA3.1(+)-MC38-TCRA-30-picky_sticky TCRA (Mus musculus) Mammalian Expression
138984 pcDNA3.1(+)-MC38-TCRB-30-picky_sticky TCRB (Mus musculus) Mammalian Expression
138985 pcDNA3.1(+)-MC38-TCRA-31-murderous_crow TCRA (Mus musculus) Mammalian Expression
138986 pcDNA3.1(+)-MC38-TCRB-31-murderous_crow TCRB (Mus musculus) Mammalian Expression
138987 pcDNA3.1(+)-MC38-TCRA-32-riot_punisher TCRA (Mus musculus) Mammalian Expression
138988 pcDNA3.1(+)-MC38-TCRB-32-riot_punisher TCRB (Mus musculus) Mammalian Expression
138989 pcDNA3.1(+)-MC38-TCRA-33-MC_hammer TCRA (Mus musculus) Mammalian Expression
138990 pcDNA3.1(+)-MC38-TCRB-33-MC_hammer TCRB (Mus musculus) Mammalian Expression

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Explore an alternative way to clone TILs by first single-cell TCR-seq'ing them and then transforming non-exhausted T cells with these TIL TCRs

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