Compare mRNA transfer with DNA transfer

[hammer]

Alex Marson indicated that his lab transfers TCRs as DNA rather than mRNA; it could be a fun project to compare his protocol with ours, perhaps in a separate work. It's also a good excuse to try to get Alex as a co-author on our paper, if we think that would be a good idea.

[pinarden]

Maybe they're replacing the endogenous TCR by a knock-in experiment and if that's the case, they need to deliver the TCRs that they're interested in, in DNA form. Because we're not knocking out the endogenous TCRs and because plasmid electroporation causes higher cytotoxicity compared to mRNA electroporation, I think for our system mRNA is still preferred. I'll try to read their recent papers and see what they did. Thanks for the notes!