Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Add matched WT to pVACfuse predictions #993

Open
susannasiebert opened this issue Jul 6, 2023 · 0 comments
Open

Add matched WT to pVACfuse predictions #993

susannasiebert opened this issue Jul 6, 2023 · 0 comments
Labels
Milestone

Comments

@susannasiebert
Copy link
Contributor

susannasiebert commented Jul 6, 2023

In pVACseq we require at least 4 overlapping amino acids between a matched WT and MT epitope. For inframe fusions in pVACfuse we could apply a similar approach by matching epitopes on the left side of the fusion to the 5" transcript and epitopes to the right of the fusion to the 3" transcript. For frameshift fusion we can match epitopes on the left side of the fusion to the 5" transcript, similar to frameshift variants in pVACseq. We would require at least 4 overlapping AAs on one end or the other; otherwise there wouldn’t be a matched WT.

This would require us to look up WT transcripts of the 5" and 3" transcripts at runtime. I believe Ensembl has an API for that (see https://rest.ensembl.org/documentation/info/sequence_id and https://ensemblrest.readthedocs.io/en/latest/#). We could probably also use the peptide fasta, if provided for the reference proteome step, and use the API as a fallback.

@susannasiebert susannasiebert added this to the 5.0.0 milestone Jul 6, 2023
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
Projects
None yet
Development

No branches or pull requests

1 participant