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name: samtools_idxstats | ||
namespace: samtools | ||
description: Reports alignment summary statistics for a BAM file | ||
keywords: [stats, mapping, counts, chromosome, bam, sam, cram] | ||
links: | ||
homepage: https://www.htslib.org/ | ||
documentation: https://www.htslib.org/doc/samtools-idxstats.html | ||
repository: https://github.com/samtools/samtools.git | ||
references: | ||
doi: 10.1093/bioinformatics/btp352, 10.1093/gigascience/giab008 | ||
license: MIT/Expat | ||
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argument_groups: | ||
- name: Inputs | ||
arguments: | ||
- name: "--bam" | ||
type: file | ||
description: BAM input file. | ||
- name: "--bai" | ||
type: file | ||
description: BAM index file. | ||
- name: "--fasta" | ||
type: file | ||
description: Reference file the CRAM was created with (optional). | ||
- name: Outputs | ||
arguments: | ||
- name: "--output" | ||
type: file | ||
description: File containing samtools stats output in tab-delimited format. | ||
required: true | ||
example: output.idxstats | ||
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resources: | ||
- type: bash_script | ||
path: script.sh | ||
test_resources: | ||
- type: bash_script | ||
path: test.sh | ||
- type: file | ||
path: test_data | ||
engines: | ||
- type: docker | ||
image: quay.io/biocontainers/samtools:1.3.1--h9071d68_10 | ||
setup: | ||
- type: docker | ||
run: | | ||
echo "$(samtools --version 2>&1)" > /var/software_versions.txt | ||
runners: | ||
- type: executable | ||
- type: nextflow |
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``` | ||
samtools idxstats | ||
``` | ||
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Usage: samtools idxstats <in.bam> |
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#!/bin/bash | ||
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## VIASH START | ||
## VIASH END | ||
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set -e | ||
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output_file="$par_output" | ||
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samtools idxstats \ | ||
"$par_bam" \ | ||
> "$output_file" |
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#!/bin/bash | ||
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echo ">>> Testing $meta_functionality_name" | ||
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"$meta_executable" \ | ||
--bam "$meta_resources_dir/test_data/a.sorted.bam" \ | ||
--bai "$meta_resources_dir/test_data/a.sorted.bam.bai" \ | ||
--output "$meta_resources_dir/test_data/a.sorted.idxstats" | ||
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echo ">>> Checking whether output exists" | ||
[ ! -f "$meta_resources_dir/test_data/a.sorted.idxstats" ] && echo "File 'a.sorted.idxstats' does not exist!" && exit 1 | ||
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echo ">>> Checking whether output is non-empty" | ||
[ ! -s "$meta_resources_dir/test_data/a.sorted.idxstats" ] && echo "File 'a.sorted.idxstats' is empty!" && exit 1 | ||
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echo ">>> Checking whether output is correct" | ||
diff "$meta_resources_dir/test_data/a.sorted.idxstats" "$meta_resources_dir/test_data/a_ref.sorted.idxstats" || \ | ||
(echo "Output file a.sorted.idxstats does not match expected output" && exit 1) | ||
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rm "$meta_resources_dir/test_data/a.sorted.idxstats" | ||
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############################################################################################ | ||
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echo ">>> Testing $meta_functionality_name with singletons in the input" | ||
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"$meta_executable" \ | ||
--bam "$meta_resources_dir/test_data/test.paired_end.sorted.bam" \ | ||
--bai "$meta_resources_dir/test_data/test.paired_end.sorted.bam.bai" \ | ||
--output "$meta_resources_dir/test_data/test.paired_end.sorted.idxstats" | ||
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echo ">>> Checking whether output exists" | ||
[ ! -f "$meta_resources_dir/test_data/test.paired_end.sorted.flagidxstatsstat" ] && echo "File 'test.paired_end.sorted.idxstats' does not exist!" && exit 1 | ||
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echo ">>> Checking whether output is non-empty" | ||
[ ! -s "$meta_resources_dir/test_data/test.paired_end.sorted.idxstats" ] && echo "File 'test.paired_end.sorted.idxstats' is empty!" && exit 1 | ||
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echo ">>> Checking whether output is correct" | ||
diff "$meta_resources_dir/test_data/test.paired_end.sorted.idxstats" "$meta_resources_dir/test_data/test_ref.paired_end.sorted.idxstats" || \ | ||
(echo "Output file test.paired_end.sorted.idxstats does not match expected output" && exit 1) | ||
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rm "$meta_resources_dir/test_data/test.paired_end.sorted.idxstats" | ||
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echo "All tests succeeded!" | ||
exit 0 |
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xx 20 6 0 | ||
* 0 0 0 |
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#!/bin/bash | ||
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# dowload test data from snakemake wrapper | ||
if [ ! -d /tmp/idxstats_source ]; then | ||
git clone --depth 1 --single-branch --branch master https://github.com/snakemake/snakemake-wrappers.git /tmp/idxstats_source | ||
fi | ||
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cp -r /tmp/idxstats_source/bio/samtools/idxstats/test/mapped/* src/samtools/idxstats/test_data | ||
# samtools idxstats a.sorted.bam > a.sorted.idxstats | ||
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# dowload test data from nf-core module | ||
wget https://github.com/nf-core/test-datasets/raw/modules/data/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam | ||
wget https://github.com/nf-core/test-datasets/raw/modules/data/genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam.bai | ||
# samtools idxstats test.paired_end.sorted.bam > test_ref.paired_end.sorted.idxstats |
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MT192765.1 29829 197 3 | ||
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