Requirements

1. there should be an individual plot for each parameter in the InteropSummary table
   • AND I WOULD SAY THAT 6 UP SHOULD DO THE JOB
2. plot points which fail the Westgard rules should be indicated on the plots by being circled
   • BIG HONKING CIRCLES IN RED!
3. the start date for runs to be included in the report should be a configurable parameter
   • (AND IT LOOKS LIKE WE GAINED CONTROL IN NOVEMBER, SO LETS START FROM THEN FOR NOW)
4. means and standard deviations should be calculated from the runs since the start date only
5. there should be a list of reagents, and the number of days until they expire (taken from the expiration date of the last used reagent)
6. there should be a line at the top of the page with information about the current run
7. the mean, median, and standard deviation of each parameter should be displayed in a separate table
8. there should be a graph of "Cluster Density", as an absolute number, with horizontal lines at 500 and 1200
9. there should be a graph of "Bases passing Q30 cutoffs", as a percentage, including both read 1 and read 2
10. the report should indicate if there are any reagents expiring within one month
11. the report should indicate if there are any runs for which data is not available
12. the cutoff date should be displayed at the top of the page
13. parameters which are stored as proportions in the database should be displayed as percentages
14. the intensity parameters (INT___*) should be displayed last in the plots and parameter table
15. we should use the following Westgard multirules (http://www.westgard.com/mltirule.htm):
    • 1x3s ie 1x > 3 sigma (either way)
    • 2x2s ie 2x > 2 sigma (consecutively in the same direction)
    • 4x1s (ie 4 in a row >1 sigma in same direction)
    • R4s (a change from <-2 sigma to >2 sigma, or vice versa)
    • 10x (10 or more consecutive results on one side of the mean value)
16. we should also have a footnote that provides the names of the five rules being used, as well as the absolute tolerances

• emphasis that this needs to be a footnote, at the BOTTOM of the page

17. we should display the ± 1 sigma in dotted light green, the ± 2 sigma in dotted yellow and the ±3 sigma as a solid red line