Add motion correction for Widefield Imaging

[weiglszonja]

Motion Correction for Widefield raw imaging

• For each .ome.tif file there is a corresponding .mcorr_1.mat file which contains the x, and y shifts for each frame.
• We are saving the x, and y shifts (which ought to be in pixels, though the example data that we received only contains 0s and 1s) as TimeSeries
• I am using the frame indices from the raw imaging data to add the TimeSeries of the y and x shifts for the blue and violet frames separately
• The motion corrected video is not saved by the Pinto lab, they are only saving the x, and y shifts and computing the motion correction on the fly

DrChicken_20230419_20hz.nwb

[CodyCBakerPhD]

Does the dtype of the MotionCorrection data automatically scale? I notice because in the example file it's uint8 but the image size is 1024 x 1024 so, IDK, it might be conceptually possible for a shift factor to be larger than the 256 bound of uint8?

[weiglszonja]

Does the dtype of the MotionCorrection data automatically scale? I notice because in the example file it's uint8 but the image size is 1024 x 1024 so, IDK, it might be conceptually possible for a shift factor to be larger than the 256 bound of uint8?

It's hard to now without seeing "actual" pixel values, but I guess it could be possible. So set it to uint16?