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luiztauffer committed Dec 12, 2024
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title: Exploring data with Neurosift
description: >
Lorem ipsum dolor sit amet...
authors: [luiztauffer, bendichter]
author: Luiz Tauffer ([@luiztauffer](https://github.com/luiztauffer)) and Ben Dichter ([@bendichter](https://github.com/bendichter))
tags: [hola]
date: 2018-01-04
---
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---
title: Unraveling Anesthesia's Effect on Brain Activity
authors: [pauladkisson]
author: Paul Adkisson ([@pauladkisson](https://github.com/pauladkisson))
description: >
Have you ever wondered what happens to your brain when you're under anesthesia? What neural changes coincide with that dramatic loss of consciousness? In this post, we'll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive's [Dandiset #000458](https://dandiarchive.org/dandiset/000458).
tags: [ reuse-showcase ]
date: 2024-11-10
---

_Data Reuse Highlight: This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI._
{{< alert title="Data Reuse Highlight:" >}}This blog entry is part of an ongoing series that showcases examples of data reuse and reanalysis using publicly available data on DANDI.{{< /alert >}}

Have you ever wondered what happens to your brain when you're under anesthesia? What neural changes coincide with that dramatic loss of consciousness?
In this post, we'll explore how two different research teams investigated this fascinating question using the same data from DANDI Archive's [Dandiset #000458](https://dandiarchive.org/dandiset/000458).

{/* truncate */}

## The Original Study: Peering into the Anesthetized Brain
In June 2023, Dr. Leslie Claar and colleagues from the Allen Institute published their groundbreaking findings in the journal _eLife_ in the article, [“Cortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice”](https://doi.org/10.7554/eLife.84630.3).
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After their experiments, Claar and her team published their data on DANDI, ensuring that it would remain publicly accessible for any future researchers who wanted to use it. The dataset and manuscript were prepared and published concurrently, which ensured that the dataset included all the paper-relevant data and metadata and curious reviewers could inspect the data directly if they so wished.

![Figure1](/img/blog/data_reuse_000458_elife-fig7.jpg)
![Figure1](./data_reuse_000458_elife-fig7.jpg)

_**Figure 1: Brain state modulates the ERP via cortico-thalamo-cortical interactions.**
(A) Butterfly plot of ERPs during non-running (quiet wakefulness), running (active wakefulness), and isoflurane-anesthetized states.
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Conversely, setting it to the hyperpolarizing regime (-80mV) mimicked the responses seen in anesthetized mice (Figure 2 B&F right).
This data provides evidence for the hypothesized bidirectional relationship.

![Figure2](/img/blog/data_reuse_000458_cell-fig4.jpg)
![Figure2](./data_reuse_000458_cell-fig4.jpg)

_**Figure 2: Shunting inhibition promotes local network desynchronization and response flexibility.**
(A) High-density Neuropixels (NPXs) recordings were used to compare spiking activity in the same cortical neurons under awake and anesthetized conditions.
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