miMAP is a tool for allowing microRNA meta analysis. Users can download public dataset (e.g., from the Sequence Read Archive (SRA) directly processable in the tool or provide their own data. Once the sequences are extracted from the .sra archive they are made compatibale to work in QIIME, then the following steps occur:
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Performing a closed-reference miRNAs picking against a modified version of the Human Ensemble non-coding RNA database without the miRNAs annotation.
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Filtering from the original sequences file all the sequences that did not hit the Human Ensemble non-coding RNA database.
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Performing another closed-reference miRNAs picking with the filtered sequences from step 2. against the miRBASE mature miRNAs database
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Making a miRNA table from step 3.
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Computing principal coordinate analysis with Bray Curtis
miMAP consists of pyqi and python code and use some external applications.