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microns_phase3_nda

nda schema for MICrONS phase3 animal 21617. For more on the MICrONS project please see: MICrONS Explorer

Technical documentation

Technical documentation on the functional data can be found here (link pending).

Installation Instructions

This package requires access to the functional data. To download the SQL database get started on the microns-nda-access repo here.

Import Instructions

Import datajoint. Configuration instructions: https://docs.datajoint.io/python/setup/01-Install-and-Connect.html

import datajoint as dj

Using the schema

To view schema ERD:

dj.ERD(nda)

nda

Tutorial:

Using DataJoint to Access Functional Data Tutorial

nda table descriptions

nda.Scan: Information on completed scans. Cajal Pipeline: meso.ScanInfo

nda.Field: Individual fields of scans. Cajal Pipeline: meso.ScanInfo.Field

nda.RasterCorrection: Digital correction for bidirectional scanning alignment. Cajal Pipeline:meso.RasterCorrection

nda.MotionCorrection: Digital correction for lateral movement of the tissue beneath the objective. Cajal Pipeline: meso.MotionCorrection

nda.ScanTimes: Timestamps of scan frames in seconds relative to the start of the scan for the first pixel of the first imaging field.

nda.MeanIntensity: Mean intensity of imaging field over time. Cajal Pipeline: meso.Quality.MeanIntensity

nda.SummaryImages: Reference images of the scan field. Cajal Pipeline: meso.SummaryImages

nda.Segmentation: CNMF segmentation of a field using CaImAn package (https://github.com/simonsfoundation/CaImAn). It records the masks of all segmented cells. Mask_id's are unique per field. Cajal Pipeline: meso.Segmentation.Mask

nda.Fluorescence: Records the raw fluorescence traces for each segmented mask. Cajal Pipeline: meso.Fluorescence.Trace

nda.ScanUnit: Unit_id assignment that is unique across the entire scan. Includes info about each unit. Cajal Pipeline: meso.ScanSet.Unit / meso.ScanSet.UnitInfo

nda.UnitHash: Hash assignment to each unique session - scan_idx - unit_id triplet.

nda.Activity: Deconvolved spike trace from the fluorescence trace. Cajal Pipeline: meso.Activity.Trace

nda.MaskClassification: Classification of segmented masks into soma or artifact. Uses CaImAn package (https://github.com/simonsfoundation/CaImAn). Cajal Pipeline: meso.MaskClassification.Type

nda.Oracle: Leave-one-out correlation for repeated videos in stimulus.

nda.Stack: High-res anatomical stack information. Cajal Pipeline: stack.CorrectedStack

nda.Registration: Parameters of the affine matrix learned for field registration into the stack. Cajal Pipeline: stack.Registration.Affine

nda.StackUnit: Unit coordinates in stack reference frame after field registration. meso.StackCoordinates.UnitInfo

nda.Stimulus: For each scan, contains the movie aligned to activity traces in nda.Activity.

nda.Trial: Contains information for each trial of the movie in nda.Stimulus. There are three types of trials, Clip, Monet2, and Trippy. Each unique trial has its own condition_hash. To get detailed information for each trial stimulus, join each condition_hash according to its corresponding type in one of: nda.Clip, nda.Monet2, or nda.Trippy.

nda.Clip: Detailed information for movie clips.

nda.Monet2: Detailed information for the Monet2 stimulus.

nda.Trippy: Detailed information for the Trippy stimulus.

nda.RawDLCPupil: Pupil traces for each scan from the left eye collected at ~20 Hz and automatically tracked in DeepLabCut. Cajal Pipeline: Pupil.Eye and Pupil.FittedPupil.Ellipse

nda.DLCPupil: Manual pupil traces low-pass filtered with a hamming window to the scan frame rate and linearly interpolated to scan frame times.

nda.RawTreadmill: Cylindrical treadmill rostral-caudal position extracted with a rotary optical encoder at ~100Hz and converted into velocity. Cajal Pipeline: treadmill.Treadmill

nda.Treadmill: Treadmill velocities low-pass filtered with a hamming window to the scan frame rate then linearly interpolated to scan frame times.

For more documentation see: Cajal Pipeline Documentation

Acknowledgement of Government Sponsorship

Supported by the Intelligence Advanced Research Projects Activity (IARPA) via Department of Interior / Interior Business Center (DoI/IBC) contract number D16PC00003. The U.S. Government is authorized to reproduce and distribute reprints for Governmental purposes notwithstanding any copyright annotation thereon. Disclaimer: The views and conclusions contained herein are those of the authors and should not be interpreted as necessarily representing the official policies or endorsements, either expressed or implied, of IARPA, DoI/IBC, or the U.S. Government.

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functional data release for animal 21617

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