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Merge pull request #286 from neuropoly/bep031
Examples for Microscopy BEP031 Recently merged validator PR for BEP031, it is working as expected with the two new examples.
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1 mouse spinal cord sample imaged over 2 sessions. | ||
Example dataset with empty images containing scanning electron microscopy (SEM) data to illustrate BIDS convention. | ||
|
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{ | ||
"Name": "micr_SEM", | ||
"Authors": [ | ||
"Etienne Bergeron", | ||
"Marie-Hélène Bourget", | ||
"Julien Cohen-Adad" | ||
], | ||
"BIDSVersion":"1.7.0", | ||
"License": "CC0" | ||
} |
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{ | ||
"participant_id": { | ||
"Description": "Unique participant ID" | ||
}, | ||
"sex": { | ||
"Description": "sex of the participant", | ||
"Levels": { | ||
"M": "male", | ||
"F": "female" | ||
} | ||
}, | ||
"species": { | ||
"Description": "Binomial species name from the NCBI Taxonomy (https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi)" | ||
}, | ||
"strain": { | ||
"Description": "string value indicating the strain of the species" | ||
}, | ||
"strain_rrid": { | ||
"Description": "research resource identifier of the strain of the species from https://scicrunch.org/resources/Organisms/search" | ||
} | ||
} |
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participant_id sex species strain strain_rrid | ||
sub-01 M mus musculus C57BL/6J RRID:IMSR_JAX:000664 |
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{ | ||
"sample_id": { | ||
"Description": "Sample ID" | ||
}, | ||
"participant_id": { | ||
"Description": "Participant ID from whom tissue samples have been acquired" | ||
}, | ||
"sample_type": { | ||
"Description": "Type of sample from ENCODE Biosample Type (https://www.encodeproject.org/profiles/biosample_type)" | ||
} | ||
} |
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sample_id participant_id sample_type | ||
sample-A sub-01 tissue |
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micr_SEM/sub-01/ses-01/micr/sub-01_ses-01_sample-A_SEM.json
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{ | ||
"Manufacturer": "Hamamatsu", | ||
"ManufacturersModelName": "C4742-95-12G04", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SliceThickness": 1, | ||
"TissueDeformationScaling": 98, | ||
"PixelSize": [0.18, 0.18], | ||
"PixelSizeUnits": "um" | ||
} |
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micr_SEM/sub-01/ses-02/micr/sub-01_ses-02_sample-A_SEM.json
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{ | ||
"Manufacturer": "Hamamatsu", | ||
"ManufacturersModelName": "C4742-95-12G04", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SliceThickness": 1, | ||
"TissueDeformationScaling": 98, | ||
"PixelSize": [0.18, 0.18], | ||
"PixelSizeUnits": "um" | ||
} |
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{ | ||
"session_id": { | ||
"Description": "Session ID" | ||
}, | ||
"acq_time": { | ||
"Description": "Time when the sample have been acquired" | ||
} | ||
} |
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session_id acq_time | ||
ses-01 2015-11-04T14:10:00 | ||
ses-02 2015-11-05T11:15:00 |
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2 rat spinal cord samples from the same subject with 4 chunks each. | ||
Example dataset with empty images containing single plane illumination microscopy (SPIM) data to illustrate BIDS convention. | ||
|
||
For demonstration purposes, JSON metadata includes fictional `ChunkTransformationMatrix` and `ChunkTransformationMatrixAxis` fields corresponding to the following transformation for both samples: | ||
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||
- All chunks are scaled by a factor 2 in the Y axis, | ||
- chunk-01 is at the origin | ||
- chunk-02 is translated by 5 um along X, | ||
- chunk-03 is translated by 3 um along Z, | ||
- chunk-04 is translated by 5 um along X and by 3 um along Z |
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{ | ||
"Name": "micr_SPIM", | ||
"Authors": [ | ||
"Etienne Bergeron", | ||
"Marie-Hélène Bourget", | ||
"Julien Cohen-Adad" | ||
], | ||
"BIDSVersion":"1.7.0", | ||
"License": "CC0" | ||
} |
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{ | ||
"participant_id": { | ||
"Description": "Unique participant ID" | ||
}, | ||
"sex": { | ||
"Description": "sex of the participant", | ||
"Levels": { | ||
"M": "male", | ||
"F": "female" | ||
} | ||
}, | ||
"species": { | ||
"Description": "Binomial species name from the NCBI Taxonomy (https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi)" | ||
} | ||
} |
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participant_id sex species | ||
sub-01 M rattus norvegicus |
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{ | ||
"sample_id": { | ||
"Description": "Sample ID" | ||
}, | ||
"participant_id": { | ||
"Description": "Participant ID from whom tissue samples have been acquired" | ||
}, | ||
"sample_type": { | ||
"Description": "Type of sample from ENCODE Biosample Type (https://www.encodeproject.org/profiles/biosample_type)" | ||
} | ||
} |
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sample_id participant_id sample_type | ||
sample-A sub-01 tissue | ||
sample-B sub-01 tissue |
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micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-01_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 0], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 0], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-02_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 5], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 0], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-03_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 0], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 3], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-04_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 5], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 3], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-01_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 0], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 0], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-02_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 5], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 0], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-03_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 0], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 3], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-04_SPIM.json
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{ | ||
"Manufacturer": "Miltenyi Biotec", | ||
"ManufacturersModelName": "UltraMicroscope II", | ||
"BodyPart": "CSPINE", | ||
"SampleEnvironment": "ex vivo", | ||
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde", | ||
"SampleStaining": "Luxol fast blue", | ||
"PixelSize": [1, 1, 1], | ||
"PixelSizeUnits": "um", | ||
"Immersion": "Oil", | ||
"NumericalAperture": 1.4, | ||
"Magnification": 40, | ||
"ChunkTransformationMatrix": | ||
[[1, 0, 0, 5], | ||
[0, 2, 0, 0], | ||
[0, 0, 1, 3], | ||
[0, 0, 0, 1]], | ||
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"] | ||
} |
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