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Merge pull request #286 from neuropoly/bep031
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Examples for Microscopy BEP031

Recently merged validator PR for BEP031, it is working as expected with the two new examples.
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rwblair authored Jan 12, 2022
2 parents 3e91333 + 56f6d6a commit 482cc91
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12 changes: 10 additions & 2 deletions README.md
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Expand Up @@ -12,8 +12,8 @@ files**. These datasets can be useful to:
**ALL RAW DATA FILES IN THIS REPOSITORY ARE EMPTY!**

However for some of the data, the headers containing the metadata are still
intact. (For example the NIfTI headers for `.nii` files, or the BrainVision data
headers for `.vhdr` files.)
intact. (For example the NIfTI headers for `.nii` files, the BrainVision data
headers for `.vhdr` files, or the OME-XML headers for `.ome.tif` files.)

Headers are intact for the following datasets:

Expand Down Expand Up @@ -117,6 +117,7 @@ bids-examples (in alphabetical order).
- [ASL datasets](#asl-datasets)
- [qMRI datasets](#qmri-datasets)
- [PET datasets](#PET-datasets)
- [Microscopy datasets](#microscopy-datasets)
- [Multimodal datasets](#multimodal-datasets)

### EEG datasets
Expand Down Expand Up @@ -204,6 +205,13 @@ bids-examples (in alphabetical order).
| pet004 | @mnoergaard | PET, blood | |
| pet005 | @mnoergaard | T1w, PET | |

### Microscopy datasets

| name | maintained by | description |
| ----------| ------------- | ----------------------------------------------------------------------------------------------- |
| micr_SEM | @jcohenadad | Example SEM dataset in PNG format with 1 sample imaged over 2 sessions |
| micr_SPIM | @jcohenadad | Example SPIM dataset in OME-TIFF format with 2 samples from the same subject with 4 chunks each |

### Multimodal datasets

| name | maintained by | description | mri | meg | eeg | ieeg | genetics | link to full data |
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3 changes: 3 additions & 0 deletions micr_SEM/README
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1 mouse spinal cord sample imaged over 2 sessions.
Example dataset with empty images containing scanning electron microscopy (SEM) data to illustrate BIDS convention.

10 changes: 10 additions & 0 deletions micr_SEM/dataset_description.json
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{
"Name": "micr_SEM",
"Authors": [
"Etienne Bergeron",
"Marie-Hélène Bourget",
"Julien Cohen-Adad"
],
"BIDSVersion":"1.7.0",
"License": "CC0"
}
21 changes: 21 additions & 0 deletions micr_SEM/participants.json
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{
"participant_id": {
"Description": "Unique participant ID"
},
"sex": {
"Description": "sex of the participant",
"Levels": {
"M": "male",
"F": "female"
}
},
"species": {
"Description": "Binomial species name from the NCBI Taxonomy (https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi)"
},
"strain": {
"Description": "string value indicating the strain of the species"
},
"strain_rrid": {
"Description": "research resource identifier of the strain of the species from https://scicrunch.org/resources/Organisms/search"
}
}
2 changes: 2 additions & 0 deletions micr_SEM/participants.tsv
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participant_id sex species strain strain_rrid
sub-01 M mus musculus C57BL/6J RRID:IMSR_JAX:000664
11 changes: 11 additions & 0 deletions micr_SEM/samples.json
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{
"sample_id": {
"Description": "Sample ID"
},
"participant_id": {
"Description": "Participant ID from whom tissue samples have been acquired"
},
"sample_type": {
"Description": "Type of sample from ENCODE Biosample Type (https://www.encodeproject.org/profiles/biosample_type)"
}
}
2 changes: 2 additions & 0 deletions micr_SEM/samples.tsv
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sample_id participant_id sample_type
sample-A sub-01 tissue
11 changes: 11 additions & 0 deletions micr_SEM/sub-01/ses-01/micr/sub-01_ses-01_sample-A_SEM.json
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{
"Manufacturer": "Hamamatsu",
"ManufacturersModelName": "C4742-95-12G04",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SliceThickness": 1,
"TissueDeformationScaling": 98,
"PixelSize": [0.18, 0.18],
"PixelSizeUnits": "um"
}
1 change: 1 addition & 0 deletions micr_SEM/sub-01/ses-01/micr/sub-01_ses-01_sample-A_SEM.png
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11 changes: 11 additions & 0 deletions micr_SEM/sub-01/ses-02/micr/sub-01_ses-02_sample-A_SEM.json
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{
"Manufacturer": "Hamamatsu",
"ManufacturersModelName": "C4742-95-12G04",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SliceThickness": 1,
"TissueDeformationScaling": 98,
"PixelSize": [0.18, 0.18],
"PixelSizeUnits": "um"
}
1 change: 1 addition & 0 deletions micr_SEM/sub-01/ses-02/micr/sub-01_ses-02_sample-A_SEM.png
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8 changes: 8 additions & 0 deletions micr_SEM/sub-01/sub-01_sessions.json
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{
"session_id": {
"Description": "Session ID"
},
"acq_time": {
"Description": "Time when the sample have been acquired"
}
}
3 changes: 3 additions & 0 deletions micr_SEM/sub-01/sub-01_sessions.tsv
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session_id acq_time
ses-01 2015-11-04T14:10:00
ses-02 2015-11-05T11:15:00
10 changes: 10 additions & 0 deletions micr_SPIM/README
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2 rat spinal cord samples from the same subject with 4 chunks each.
Example dataset with empty images containing single plane illumination microscopy (SPIM) data to illustrate BIDS convention.

For demonstration purposes, JSON metadata includes fictional `ChunkTransformationMatrix` and `ChunkTransformationMatrixAxis` fields corresponding to the following transformation for both samples:

- All chunks are scaled by a factor 2 in the Y axis,
- chunk-01 is at the origin
- chunk-02 is translated by 5 um along X,
- chunk-03 is translated by 3 um along Z,
- chunk-04 is translated by 5 um along X and by 3 um along Z
10 changes: 10 additions & 0 deletions micr_SPIM/dataset_description.json
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{
"Name": "micr_SPIM",
"Authors": [
"Etienne Bergeron",
"Marie-Hélène Bourget",
"Julien Cohen-Adad"
],
"BIDSVersion":"1.7.0",
"License": "CC0"
}
15 changes: 15 additions & 0 deletions micr_SPIM/participants.json
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{
"participant_id": {
"Description": "Unique participant ID"
},
"sex": {
"Description": "sex of the participant",
"Levels": {
"M": "male",
"F": "female"
}
},
"species": {
"Description": "Binomial species name from the NCBI Taxonomy (https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi)"
}
}
2 changes: 2 additions & 0 deletions micr_SPIM/participants.tsv
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participant_id sex species
sub-01 M rattus norvegicus
11 changes: 11 additions & 0 deletions micr_SPIM/samples.json
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{
"sample_id": {
"Description": "Sample ID"
},
"participant_id": {
"Description": "Participant ID from whom tissue samples have been acquired"
},
"sample_type": {
"Description": "Type of sample from ENCODE Biosample Type (https://www.encodeproject.org/profiles/biosample_type)"
}
}
3 changes: 3 additions & 0 deletions micr_SPIM/samples.tsv
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sample_id participant_id sample_type
sample-A sub-01 tissue
sample-B sub-01 tissue
19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-01_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 0],
[0, 2, 0, 0],
[0, 0, 1, 0],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-02_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 5],
[0, 2, 0, 0],
[0, 0, 1, 0],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-03_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 0],
[0, 2, 0, 0],
[0, 0, 1, 3],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-A_stain-LFB_chunk-04_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 5],
[0, 2, 0, 0],
[0, 0, 1, 3],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-01_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 0],
[0, 2, 0, 0],
[0, 0, 1, 0],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-02_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 5],
[0, 2, 0, 0],
[0, 0, 1, 0],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-03_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 0],
[0, 2, 0, 0],
[0, 0, 1, 3],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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19 changes: 19 additions & 0 deletions micr_SPIM/sub-01/micr/sub-01_sample-B_stain-LFB_chunk-04_SPIM.json
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{
"Manufacturer": "Miltenyi Biotec",
"ManufacturersModelName": "UltraMicroscope II",
"BodyPart": "CSPINE",
"SampleEnvironment": "ex vivo",
"SampleFixation": "4% paraformaldehyde, 2% glutaraldehyde",
"SampleStaining": "Luxol fast blue",
"PixelSize": [1, 1, 1],
"PixelSizeUnits": "um",
"Immersion": "Oil",
"NumericalAperture": 1.4,
"Magnification": 40,
"ChunkTransformationMatrix":
[[1, 0, 0, 5],
[0, 2, 0, 0],
[0, 0, 1, 3],
[0, 0, 0, 1]],
"ChunkTransformationMatrixAxis": ["X", "Y", "Z"]
}
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