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demultiplex failure #49
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I modified manifest file in:
But I obtained always same results. |
If you increase the "demultiplex_min_reads" even more? I set mine to 50000. |
@martinaryee @vedtopkar It's unfortunately clearly broken. It would be highly appreciated if you could take a look at the demultiplexing step again. I have the same errors as @Salvobioinfo . |
@Zethson In the first our attempt the issue was due to the sequencing problems, Indeed I tried to demultiplex our data with different tools and only one sample has a good reads amount. |
Running command line:
python guideseq.py all -m manifest_1.yaml
after demultiplexing begins I receive:
IOError: [Errno 24] Too many open files: ...
Upon inspection of the demultiplexed output directory, there is one file for every barcode.
I saw other issue like mine, but others solutions don't work for me.
My manifest file is:
Any idea what is going on here?
Salvatore
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