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Merge pull request #238 from aehrc/load_vcf_bgz
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Load bgzipped VCF files
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bhosking authored Apr 15, 2024
2 parents d14dcb7 + 47dda8f commit 2fb2642
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30 changes: 30 additions & 0 deletions src/main/java/au/csiro/variantspark/utils/FileUtils.java
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package au.csiro.variantspark.utils;

import java.io.*;
import java.util.zip.GZIPInputStream;
import java.io.IOException;
import htsjdk.samtools.util.BlockCompressedInputStream;

public class FileUtils {

/**
*
* @param file: an input file
* @return true if input file is BGZIP by check the first two byte of input file
*/
public static boolean isBGZFile(String filePath) {
/**
* .vcf.bgz is type of GZP file, work well with BlockCompressedInputStream
* .vcf.gz is also GZP file but get java.lang.OutOfMemoryError at java.io.InputStreamReader.read(InputStreamReader.java:184)
* .vcf.bz2 is not GZP file and get java.lang.OutOfMemoryError at java.io.InputStreamReader.read(InputStreamReader.java:184)
* .vcf is not GZP file and get htsjdk.samtools.SAMFormatException: at header from java.io.BufferedReader.readLine(BufferedReader.java:389)
*/
try (BufferedInputStream bufferedInputStream = new BufferedInputStream(new FileInputStream(filePath))) {
boolean isValid = BlockCompressedInputStream.isValidFile(bufferedInputStream);
return isValid;
} catch (IOException e) {
//handle exception for non proper bgzip file
return false;
}
}
}
23 changes: 20 additions & 3 deletions src/main/scala/au/csiro/variantspark/cli/args/SparkArgs.scala
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Expand Up @@ -2,15 +2,32 @@ package au.csiro.variantspark.cli.args

import org.kohsuke.args4j.Option
import au.csiro.pbdava.ssparkle.spark.SparkApp
import au.csiro.variantspark.utils.FileUtils
import org.apache.spark.rdd.RDD
import htsjdk.samtools.util.BlockCompressedInputStream
import org.apache.hadoop.fs.Path
import java.io.File

trait SparkArgs extends SparkApp {

@Option(name = "-sp", required = false, usage = "Spark parallelism (def=<default-spark-par>)",
aliases = Array("--spark-par"))
val sparkPar: Int = 0

def textFile(inputFile: String): RDD[String] =
sc.textFile(inputFile, if (sparkPar > 0) sparkPar else sc.defaultParallelism)

def textFile(inputFile: String): RDD[String] = {
val isBGZ = FileUtils.isBGZFile(inputFile)
println(inputFile + " is loading to spark RDD, isBGZFile: " + isBGZ)
if (isBGZ) {
// BGZIP file is compressed as blocks, requires specialized libraries htsjdk
val path = new Path(inputFile)
val fs = path.getFileSystem(sc.hadoopConfiguration)
val bgzInputStream = new BlockCompressedInputStream(fs.open(path))
// each blocks can be decompressed independently and to be read in parallel
sc.parallelize(Stream.continually(bgzInputStream.readLine()).takeWhile(_ != null).toList)
} else {
// The standard GZIP libraries can handle files compressed as a whole
// load .vcf, .vcf.gz or .vcf.bz2 to RDD
sc.textFile(inputFile, if (sparkPar > 0) sparkPar else sc.defaultParallelism)
}
}
}

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