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Docs: fixed hyphens in CHANGES.txt
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alexeigurevich committed Aug 6, 2018
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42 changes: 21 additions & 21 deletions CHANGES.txt
Original file line number Diff line number Diff line change
Expand Up @@ -25,48 +25,48 @@
specified with "--k-mer-size" option.

4. Improved and extended gene prediction/annotation functionality:
Barrnap for rRNA genes prediction ("--rna-finding") is added;
BUSCO for finding conserved single-copy orthologs ("--conserved-genes-finding";
- Barrnap for rRNA genes prediction ("--rna-finding") is added;
- BUSCO for finding conserved single-copy orthologs ("--conserved-genes-finding";
Linux only) is added;
regular predicted genes (using GeneMark or Glimmer) are split into full and partial;
"--fungus" option is added for more accurate processing of fungus assemblies using
- regular predicted genes (using GeneMark or Glimmer) are split into full and partial;
- "--fungus" option is added for more accurate processing of fungus assemblies using
GeneMark-ES and BUSCO;
"--features" option is added to replace "-G/--genes", it allows to count all genomic
- "--features" option is added to replace "-G/--genes", it allows to count all genomic
features from GFF or any specific feature type (e.g., 'CDS').

5. Icarus updates:
changes in alignment viewers:
- changes in alignment viewers:
* GC% track is added to the read coverage pane;
* a button for highlighting all assembly misassemblies is added;
* local misassemblies are now unchecked (hidden) by default.
static Circos plot of alignments ("--circos") is added;
chromosome names in the main menu are sorted in the human-friendly order now
- static Circos plot of alignments ("--circos") is added;
- chromosome names in the main menu are sorted in the human-friendly order now
(e.g., chr1, chr2, ..., chr10 instead of chr1, chr10, chr2, ...).

6. Improved reads support:
reads are now mapped to all assemblies and various alignment stats are reported;
single ("--single") and interlaced ("--12") reads are supported;
multiple read libraries are supported, including both paired-end ("--pe1/2/12")
- reads are now mapped to all assemblies and various alignment stats are reported;
- single ("--single") and interlaced ("--12") reads are supported;
- multiple read libraries are supported, including both paired-end ("--pe1/2/12")
and mate-pair ("--mp1/2/12") libraries;
Oxford Nanopores ("--nanopore") and PacBio SMRT ("--pacbio") are supported;
ready SAM and BAM files can be provided both for reads mapped against assemblies
- Oxford Nanopores ("--nanopore") and PacBio SMRT ("--pacbio") are supported;
- ready SAM and BAM files can be provided both for reads mapped against assemblies
("--sam/bam") and reads mapped against the reference genome ("--ref-sam/bam");
- reads stats can still be skipped by using "--no-read-stats" option.

7. Modified processing of undefined nucleotides ('N'):
reference Ns are excluded from Genome Fraction computation (100% if all ACGT bases
- reference Ns are excluded from Genome Fraction computation (100% if all ACGT bases
are covered);
assembly Ns are excluded from "Unaligned" and "partially unaligned length"
- assembly Ns are excluded from "Unaligned" and "partially unaligned length"
computation;
scaffold gaps are now defined as simply a gap between alignments having at least 10
- scaffold gaps are now defined as simply a gap between alignments having at least 10
consecutive Ns (affects "# scaffold gap size mis.", previously it was underestimated
due to a strict threshold on the percentage of Ns in the gap sequence).

8. MetaQUAST changes:
trying to download next best match if a reference genome is not found in NCBI
- trying to download next best match if a reference genome is not found in NCBI
(without references mode only);
- link to the combined reference report is added to the main report HTML;
sample summary reports (TXT, TEX, etc) are renamed to exclude special characters in
- sample summary reports (TXT, TEX, etc) are renamed to exclude special characters in
the filenames ('#', '%', etc).

9. Changes and new metrics related to scaffold gap size misassemblies:
Expand All @@ -84,9 +84,9 @@
misassembly-related metrics).

11. Changes in the list of embedded third-party tools:
removed: GAGE, gnuplot;
replaced: MUMmer and E-MEM (new: Minimap2), Manta (new: GRIDSS);
added: BUSCO, Barrnap, KMC, Red.
- removed: GAGE, gnuplot;
- replaced: MUMmer and E-MEM (new: Minimap2), Manta (new: GRIDSS);
- added: BUSCO, Barrnap, KMC, Red.

12. Fixed several minor bugs.

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