This tools takes a control fastq seqeuncing library and an experimental libray treeted with the endonuclease you are trying ti finda TAM /PAM site for. The two libraries are generated from a pool of sequences containing a guide site and a mixture of random bases flanking the guide region. When a endonuclease finds the PAM/TAM site anda guide RNA it cuts and those sequences are depleted in the sample library relative to the control.
To launch a the streamlit web application locally at http://localhost:8501 run this commmand:
streamlit run app.py