HOST-PATHOGEN PROTEIN-PROTEIN INTERACTION PREDICTOR (HPIPred)

This tool allows for the prediction of putative host-pathogen protein-protein interactions based on numerical encoding of physicochemical descriptors

GETTING STARTED

Pre-requisites

This tool has been tested on Ubuntu (18.04) and MacOS Catalina. Please, make sure to install local blast, R and Zenity.

Ubuntu:

Install blast locally: bash sudo apt-get install ncbi-blast+

Install R: bash sudo apt install r-base

Install Zenity: bash sudo apt install zenity

MacOS:

To install blast locally follow the instructions in: https://www.ncbi.nlm.nih.gov/books/NBK52640/

Install R package in: https://cran.r-project.org/bin/macosx/

Install Zenity*: brew install zenity

• Please note that you need homebrew installed in your computer. To install homebrew for mac follow the instructions in: https://brew.sh

Installation

Please, download the repository as a zip file and uncompress it

Download dataset/ and database/ directories* from Zenodo into the main directory where the repository is allocated in your system. Then, unzip the files.

*Dataset and database directories are available under: https://zenodo.org/record/4668840#.YHm-B-0zYVs

RUNNING EXAMPLE

In order to run the tool, go to /scripts inside the main directory and run ./main_script.bash

After this, Zenity interface will be prompted and the user will be asked to choose:

1. A host organism (either from the available database in the repository, from uniprot via its taxon ID or from a custom file)
2. A pathogen organism
3. From a set of physicochemical descriptors (either custom or default) to perform the predictive analysis
4. The False discovery rate (this is a measure of astringency, the lower the chosen value, the more astringent the analysis will be)
5. The percentage of physicochemical models that have to agree on a prediction in order to report it in the consensus interactome

LICENSE

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.