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MM and QM/MM setup for a metalloprotein using GROMACS, Chemshell and ORCA (Using the CHARMM forcefield (both in GROMACS and with Chemshell) and the ORCA code as QM code for Chemshell.)

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Chemshell-QM/MM for complex metalloproteins (old TCl-based version)

Scripts to perform a) GROMACS MM setup and MM simulations for a metalloprotein and b) subsequent Chemshell QM/MM calculations as used in our nitrogenase QM/MM studies:

  1. QM/MM Study of the Nitrogenase MoFe Protein Resting State: Broken-Symmetry States, Protonation States, and QM Region Convergence in the FeMoco Active Site Bardi Benediktsson and Ragnar Bjornsson, Inorg. Chem. 2017, 56, 13417-13429. http://pubs.acs.org/doi/full/10.1021/acs.inorgchem.7b02158
  2. QM/MM calculations reveal a bridging hydroxo group in a vanadium nitrogenase crystal structure Bardi Benediktsson, Albert T. Thorhallsson, Ragnar Bjornsson* Chem. Comm., 2018, 54, 7310-7313.
  3. Resolving the structure of the E1 state of Mo Nitrogenase through Mo and Fe K-edge EXAFS and QM/MM calculations C. Van Stappen, A. Th. Thorhallsson, L. Decamps, R. Bjornsson*, S. DeBeer*, Chem. Sci. 2019, 10, 9807-9821.
  4. A model for dinitrogen binding in the E4 state of nitrogenase Albert Th. Thorhallsson, Bardi Benediktsson, Ragnar Bjornsson*, Chem. Sci. 2019, 10, 11110-11124.
  5. Quantum Mechanics/Molecular Mechanics Study of Resting-State Vanadium Nitrogenase: Molecular and Electronic Structure of the Iron–Vanadium Cofactor Bardi Benediktsson and Ragnar Bjornsson, Inorg. Chem. 2020, 59, 11514–11527.
  6. The E2 state of FeMoco: hydride formation vs. Fe reduction and a mechanism for H2 evolution A. Th. Thorhallsson, R. Bjornsson, Chem. Eur. J. 2021, 27, 16788-16800.
  7. Carbon Monoxide Binding to the Iron-Molybdenum Cofactor of Nitrogenase: A Detailed Quantum Mechanics/Molecular Mechanics Investigation N. B. Spiller, R. Bjornsson, S. DeBeer, F. Neese, Inorg. Chem. 2021, 60, 18031-18047.
  8. Analysis of the geometric and electronic structure of spin-coupled iron-sulfur dimers with broken-symmetry DFT: implications for FeMoco B. Benediktsson, R. Bjornsson, J. Chem. Theory Comput. 2022, accepted.

See page: https://sites.google.com/site/ragnarbjornsson/mm-and-qm-mm-setup for detailed descriptions on using the scripts.

If you find these scripts useful, please consider citing the article:

  1. QM/MM Study of the Nitrogenase MoFe Protein Resting State: Broken-Symmetry States, Protonation States, and QM Region Convergence in the FeMoco Active Site Bardi Benediktsson and Ragnar Bjornsson, Inorg. Chem. 2017, 56, 13417-13429. http://pubs.acs.org/doi/full/10.1021/acs.inorgchem.7b02158

NOTE :

Our research group has now switched over to our own open-source QM/MM program ASH which is Python-based and more convenient: See https://github.com/RagnarB83/ash and https://ash.readthedocs.io

See QM/MM tutorials: https://ash.readthedocs.io/en/latest/QM-MM-protein.html and https://ash.readthedocs.io/en/latest/Metalloprotein-I.html

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MM and QM/MM setup for a metalloprotein using GROMACS, Chemshell and ORCA (Using the CHARMM forcefield (both in GROMACS and with Chemshell) and the ORCA code as QM code for Chemshell.)

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