Skip to content

Commit

Permalink
Merge pull request #485 from Proteobench/DIA-modules-documentation
Browse files Browse the repository at this point in the history
update module documentation
  • Loading branch information
RobbinBouwmeester authored Dec 9, 2024
2 parents 4428eb0 + 0261aa6 commit 32bb444
Show file tree
Hide file tree
Showing 12 changed files with 22 additions and 16 deletions.
Original file line number Diff line number Diff line change
Expand Up @@ -213,7 +213,7 @@ Example: when 3 is selected, only the precursor ions quantified in 3 or more raw

To make the results available to the entire community, you need to provide the parameter file that corresponds to
your analysis. You can upload it in the drag and drop area in the "Add results to online repository" section (under Download calculated ratio's).
See [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-DDA-Quantification-ion-level/#important-tool-specific-settings)
See [here](#important-tool-specific-settings)
for all compatible parameter files.
In this module, we keep track of the following parameters, if you feel
that some important information is missing, please add it in the
Expand Down
Original file line number Diff line number Diff line change
@@ -1,7 +1,7 @@
# DDA quantification - peptidoforms

This module compares the sensitivity and quantification accuracy for data acquired with data-dependent acquisition (DDA) on a Q Exactive HF-X Orbitrap (Thermo Fisher).
It is based on the DDA quantification module "ion-level" described [here](https://proteobench.readthedocs.io/en/stable/available-modules/3-DDA-Quantification-peptidoform-level). Data and metrics are the same, the difference is that in this module we focus on peptidoform quantities summarised from the ion quantities.
It is based on the DDA quantification module "ion-level" described [here](#2-quant-lfq-ion-dda). Data and metrics are the same, the difference is that in this module we focus on peptidoform quantities summarised from the ion quantities.

## How to use

Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -203,7 +203,7 @@ Example: when 3 is selected, only the precursor ions quantified in 3 or more raw

To make the results available to the entire community, you need to provide the parameter file that corresponds to
your analysis. You can upload it in the drag and drop area in the "Add results to online repository" section (under Download calculated ratio's).
See [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-DDA-Quantification-ion-level/#important-tool-specific-settings)
See [here](#important-tool-specific-settings)
for all compatible parameter files.
In this module, we keep track of the following parameters, if you feel
that some important information is missing, please add it in the
Expand Down
6 changes: 6 additions & 0 deletions docs/available-modules/5-quant-lfq-ion-dia-diapasef.md
Original file line number Diff line number Diff line change
@@ -0,0 +1,6 @@
# DIA quantification - ion level - diaPASEF

This module compares the sensitivity and quantification accuracy for data acquired with data-independent acquisition (DIA) on a timsTOF (Bruker).
It is similar to the DIA quantification module "ion-level" described [here](#4-quant-lfq-ion-dia-aif).

**We are working on the documentation: more information comming soon.**
Original file line number Diff line number Diff line change
@@ -1,3 +1,3 @@

Please upload the output of your analysis, and indicate what software
tool it comes from (this is necessary to correctly parse your table - find more information in the "[How to use](https://proteobench.readthedocs.io/en/latest/available-modules/2-DDA-Quantification-ion-level/#how-to-use)" section of this module. For the data to run your workflow on please see the section "[Data set](https://proteobench.readthedocs.io/en/latest/available-modules/2-DDA-Quantification-ion-level/#data-set)". You can download an (*incomplete*) MaxQuant file example [here](https://raw.githubusercontent.com/Proteobench/ProteoBench/refs/heads/main/test/data/dda_quant/MaxQuant_2_5_1_evidence_sample.txt).
tool it comes from (this is necessary to correctly parse your table - find more information in the "[How to use](https://proteobench.readthedocs.io/en/latest/available-modules/2-quant-lfq-ion-dda/#how-to-use)" section of this module. For the data to run your workflow on please see the section "[Data set](https://proteobench.readthedocs.io/en/latest/available-modules/2-quant-lfq-ion-dda/#data-set)". You can download an (*incomplete*) MaxQuant file example [here](https://raw.githubusercontent.com/Proteobench/ProteoBench/refs/heads/main/test/data/dda_quant/MaxQuant_2_5_1_evidence_sample.txt).
Original file line number Diff line number Diff line change
Expand Up @@ -17,4 +17,4 @@ ProteoBench plots these three metrics to visualize workflow outputs
from different tools, with different versions, and/or different
sets of parameters for the search and quantification.
The full description of the pre-processing steps and metrics
calculation is available [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-DDA-Quantification-ion-level/).
calculation is available [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-quant-lfq-ion-dda/).
Original file line number Diff line number Diff line change
Expand Up @@ -2,7 +2,7 @@

To do so, you need to provide the parameter file that corresponds to
your analysis. You can upload it in the drag and drop area below.
See [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-DDA-Quantification-ion-level/)
See [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-quant-lfq-ion-dda/)
for all compatible parameter files.
In this module, we keep track of the following parameters, if you feel
that some important information is missing, please add it in the
Expand Down
Original file line number Diff line number Diff line change
@@ -1,9 +1,9 @@
Please upload the ouput of your analysis, and indicate what software
tool it comes from (this is necessary to correctly parse your table - find
more information in the "[How to use](https://proteobench.readthedocs.io/en/stable/available-modules/4-DIA-Quantification/)"
more information in the "[How to use](https://proteobench.readthedocs.io/en/stable/available-modules/4-quant-lfq-ion-dia-aif/)"
section of this module).

Currently, we support output files from AlphaDIA, DIA-NN, FragPipe, MSAID, MaxQuant and Spectronaut. It is also possible to reformat your data in a tab-delimited file that we call "custom format" in the [documentation](https://proteobench.readthedocs.io/en/latest/available-modules/4-DIA-Quantification/).
Currently, we support output files from AlphaDIA, DIA-NN, FragPipe, MSAID, MaxQuant and Spectronaut. It is also possible to reformat your data in a tab-delimited file that we call "custom format" in the [documentation](https://proteobench.readthedocs.io/en/latest/available-modules/4-quant-lfq-ion-dia-aif/).

Remember: contaminant sequences are already present in the fasta file
associated to this module. **Do not add other contaminants** to your
Expand Down
Original file line number Diff line number Diff line change
@@ -1,9 +1,9 @@
Please upload the ouput of your analysis, and indicate what software
tool it comes from (this is necessary to correctly parse your table - find
more information in the "[How to use](https://proteobench.readthedocs.io/en/stable/available-modules/4-DIA-Quantification/)"
more information in the "[How to use](https://proteobench.readthedocs.io/en/stable/available-modules/4-quant-lfq-ion-dia-aif/)"
section of this module).

Currently, we support output files from AlphaDIA and DIA-NN. It is also possible to reformat your data in a tab-delimited file that we call "custom format" in the [documentation](https://proteobench.readthedocs.io/en/latest/available-modules/4-DIA-Quantification/). We are still working on EncyclopeDIA, MSFraggerDIA, Skyline and Spectronaut.
Currently, we support output files from AlphaDIA and DIA-NN. It is also possible to reformat your data in a tab-delimited file that we call "custom format" in the [documentation](https://proteobench.readthedocs.io/en/latest/available-modules/4-quant-lfq-ion-dia-aif/). We are still working on EncyclopeDIA, MSFraggerDIA, Skyline and Spectronaut.

Remember: contaminant sequences are already present in the fasta file
associated to this module. **Do not add other contaminants** to your
Expand Down
Original file line number Diff line number Diff line change
@@ -1,3 +1,3 @@

Please upload the output of your analysis, and indicate what software
tool it comes from (this is necessary to correctly parse your table - find more information in the "[How to use](https://proteobench.readthedocs.io/en/latest/available-modules/3-DDA-Quantification-peptidoform-level/#how-to-use)" section of this module. For the data to run your workflow on please see the section "[Data set](https://proteobench.readthedocs.io/en/latest/available-modules/3-DDA-Quantification-peptidoform-level/#data-set)".
tool it comes from (this is necessary to correctly parse your table - find more information in the "[How to use](https://proteobench.readthedocs.io/en/latest/available-modules/3-quant-lfq-peptidoform-dda/#how-to-use)" section of this module. For the data to run your workflow on please see the section "[Data set](https://proteobench.readthedocs.io/en/latest/available-modules/3-quant-lfq-peptidoform-dda/#data-set)".
Original file line number Diff line number Diff line change
Expand Up @@ -2,7 +2,7 @@

To do so, you need to provide the parameter file that corresponds to
your analysis. You can upload it in the drag and drop area below.
See [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-DDA-Quantification-ion-level/)
See [here](https://proteobench.readthedocs.io/en/stable/available-modules/2-quant-lfq-ion-dda/)
for all compatible parameter files.
In this module, we keep track of the following parameters, if you feel
that some important information is missing, please add it in the
Expand Down
8 changes: 4 additions & 4 deletions webinterface/pages/texts/generic_texts.py
Original file line number Diff line number Diff line change
Expand Up @@ -8,7 +8,7 @@ class ShortMessages:

title = "DDA quantification - precursor ions"

doc_url = "https://proteobench.readthedocs.io/en/latest/available-modules/4-DIA-Quantification/"
doc_url = "https://proteobench.readthedocs.io/en/latest/available-modules/4-quant-lfq-ion-dia-aif/"

initial_results = """
Scroll down if you want to see the public benchmark runs publicly available
Expand Down Expand Up @@ -41,7 +41,7 @@ class ShortMessages:
class Help:
input_file = """
Output file of the software tool. More information on the accepted format can
be found [here](https://proteobench.readthedocs.io/en/latest/available-modules/4-DIA-Quantification/)
be found [here](https://proteobench.readthedocs.io/en/latest/available-modules/4-quant-lfq-ion-dia-aif/)
"""

pull_req = """
Expand All @@ -51,7 +51,7 @@ class Help:
input_format = """
Please select the software you used to generate the results. If it is not yet
implemented in ProteoBench, you can use a tab-delimited format that is described
further [here](https://proteobench.readthedocs.io/en/latest/available-modules/4-DIA-Quantification/)
further [here](https://proteobench.readthedocs.io/en/latest/available-modules/4-quant-lfq-ion-dia-aif/)
"""

parse_button = """
Expand All @@ -60,6 +60,6 @@ class Help:

meta_data_file = """
Please add a file with meta data that contains all relevant information about
your search parameters. See [here](https://proteobench.readthedocs.io/en/latest/available-modules/4-DIA-Quantification/)
your search parameters. See [here](https://proteobench.readthedocs.io/en/latest/available-modules/4-quant-lfq-ion-dia-aif/)
for all compatible parameter files.
"""

0 comments on commit 32bb444

Please sign in to comment.