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split, merge, reformulate assembly_visualization.md #7

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@evezeyl evezeyl commented Aug 23, 2021

Please have a review if this is satisfactory
Splitted assembly_visualization.md from BioInfoTraining.
Rechecked path on Saga, modified the organisation so each mini tutorial can be independent
check also that reference to the other tutorial work


## 2. Example: Using IGV with reads mapped to its own assembly

We have assembled reads and annotated using Bifrost pipeline. The data for today's practical are found in `/cluster/projects/nn9305k/tutorial/20210412_mapping_visualization/visualisation_files`
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remove today

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Done - but hanging on data is/are ... RRR
so "read" (on the fly) https://www.theguardian.com/news/datablog/2010/jul/16/data-plural-singular
Ok to you are ?

```bash
# transfering from SAGA
rsync -rauPW <user_name>:<source_folder> <destination_folder>
```
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Remove this, rather refer to a different file for that info

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ok, we have to merge the tutorial for file transfer then ... so created a file "transfering_files.md" - so we need to fill it
or change it to something done (did not find something only about file transfer)

Read mapping is aligning each of your reads to a/the matching locus on a longer sequence.
This longer sequence can either be the isolate assembly, or an assembly of a related isolate (eg. a reference isolate for the group of isolate under study).

You could think of read mapping as a simulation of local hybridization of your reads to the genome, at a loci where it fits well enough to hybridize. Usually few mismatches are allowed (think about the consequences). A portion of the reads may map to several loci in your genome (ie. in repeated regions).
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@karinlag karinlag Aug 30, 2021

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at a locus
Usually a few mismatches

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Maybe add that things on the ends might not match, and maybe clipping?

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Added soft and hard clipping - please review

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ok - had corrected above, like better at

mkdir mapping
cd mapping

rsync -rauPW /cluster/projects/nn9305k/tutorial/20210412_mapping_visualization/mapping_practical/* .
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refer to a page with rsync

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@evezeyl evezeyl Aug 30, 2021

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This page needs to be made. Removed this part - made a link (commented above) so has to be written

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evezeyl commented Aug 30, 2021

updated - please see if changes satisfactory.
Need to complete the filetransfer though

@novica novica changed the base branch from main to dev July 2, 2024 09:33
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novica commented Sep 19, 2024

Are people still working on this or is it stale?

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evezeyl commented Sep 19, 2024 via email

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3 participants