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readd pynapple seaborn install for dandihub users
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@stephprince
stephprince committed Apr 15, 2024
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92 changes: 72 additions & 20 deletions Workshop_2024_BCM/houston-analysis-demo.ipynb
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"source": [
"First, let's import a few classes. If you are *not* running this notebook on DANDI Hub, you will need to install these packages using `pip` or your favorite Python package manager. For example:\n",
"```\n",
"pip install dandi pynwb fsspec requests aiohttp matplotlib pynapple seaborn\n",
"pip install dandi pynwb fsspec requests aiohttp matplotlib\n",
"```"
]
},
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" <tr>\n",
" <th>0</th>\n",
" <td>MEC</td>\n",
" <td>ElectrodeGroup pynwb.ecephys.ElectrodeGroup at 0x6080627152\\nFields:\\n description: The name of the ElectrodeGroup this electrode is a part of.\\n device: EEG pynwb.device.Device at 0x6080626864\\nFields:\\n description: The device used to record EEG signals.\\n\\n location: MEC\\n</td>\n",
" <td>ElectrodeGroup pynwb.ecephys.ElectrodeGroup at 0x6004229568\\nFields:\\n description: The name of the ElectrodeGroup this electrode is a part of.\\n device: EEG pynwb.device.Device at 0x6004228800\\nFields:\\n description: The device used to record EEG signals.\\n\\n location: MEC\\n</td>\n",
" <td>ElectrodeGroup</td>\n",
" </tr>\n",
" </tbody>\n",
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" <tr>\n",
" <th>0</th>\n",
" <td>MEC</td>\n",
" <td>ElectrodeGroup pynwb.ecephys.ElectrodeGroup at 0x6080627152\\nFields:\\n description: The name of the ElectrodeGroup this electrode is a part of.\\n device: EEG pynwb.device.Device at 0x6080626864\\nFields:\\n description: The device used to record EEG signals.\\n\\n location: MEC\\n</td>\n",
" <td>ElectrodeGroup pynwb.ecephys.ElectrodeGroup at 0x6004229568\\nFields:\\n description: The name of the ElectrodeGroup this electrode is a part of.\\n device: EEG pynwb.device.Device at 0x6004228800\\nFields:\\n description: The device used to record EEG signals.\\n\\n location: MEC\\n</td>\n",
" <td>ElectrodeGroup</td>\n",
" </tr>\n",
" </tbody>\n",
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" <tr>\n",
" <th>0</th>\n",
" <td>MEC</td>\n",
" <td>ElectrodeGroup pynwb.ecephys.ElectrodeGroup at 0x6080627152\\nFields:\\n description: The name of the ElectrodeGroup this electrode is a part of.\\n device: EEG pynwb.device.Device at 0x6080626864\\nFields:\\n description: The device used to record EEG signals.\\n\\n location: MEC\\n</td>\n",
" <td>ElectrodeGroup pynwb.ecephys.ElectrodeGroup at 0x6004229568\\nFields:\\n description: The name of the ElectrodeGroup this electrode is a part of.\\n device: EEG pynwb.device.Device at 0x6004228800\\nFields:\\n description: The device used to record EEG signals.\\n\\n location: MEC\\n</td>\n",
" <td>ElectrodeGroup</td>\n",
" </tr>\n",
" </tbody>\n",
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"</table><p>... and 4 more rows.</p></details></details><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".experiment_description\">experiment_description: </span><span class=\"field-value\">The sample includes conjunctive cells and head direction cells from layers III and V of medial entorhinal cortex and have been published in Sargolini et al. (Science, 2006).</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".session_id\">session_id: </span><span class=\"field-value\">17010302</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".lab\">lab: </span><span class=\"field-value\">Moser</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".institution\">institution: </span><span class=\"field-value\">Centre for the Biology of Memory, Norwegian University of Science and Technology</span></div></div>"
],
"text/plain": [
"root pynwb.file.NWBFile at 0x6062620448\n",
"root pynwb.file.NWBFile at 0x6001542112\n",
"Fields:\n",
" acquisition: {\n",
" ElectricalSeries <class 'pynwb.ecephys.ElectricalSeries'>\n",
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" session_description: This session includes spike and position times for recorded cells from a Long Evans rat that was running in a 1 x 1 meter enclosure. The cells were recorded in the dorsocaudal 25% portion of the medial entorhinal cortex (MEC).Position is given for two LEDs to enable calculation of head direction.\n",
" session_id: 17010302\n",
" session_start_time: 1900-01-01 00:00:00+01:00\n",
" subject: subject pynwb.file.Subject at 0x6051471456\n",
" subject: subject pynwb.file.Subject at 0x6002655792\n",
"Fields:\n",
" age: P3M/P5M\n",
" age__reference: birth\n",
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" <div class='container-wrap'><div class='container-header'><div class='xr-obj-type'><h3>subject (Subject)</h3></div></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".age\">age: </span><span class=\"field-value\">P3M/P5M</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".age__reference\">age__reference: </span><span class=\"field-value\">birth</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".description\">description: </span><span class=\"field-value\">A Long Evans rat.</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".sex\">sex: </span><span class=\"field-value\">M</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".species\">species: </span><span class=\"field-value\">Rattus norvegicus</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".subject_id\">subject_id: </span><span class=\"field-value\">10073</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".weight\">weight: </span><span class=\"field-value\">0.35/0.45</span></div></div>"
],
"text/plain": [
"subject pynwb.file.Subject at 0x6051471456\n",
"subject pynwb.file.Subject at 0x6002655792\n",
"Fields:\n",
" age: P3M/P5M\n",
" age__reference: birth\n",
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" <div class='container-wrap'><div class='container-header'><div class='xr-obj-type'><h3>SpatialSeriesLED1 (SpatialSeries)</h3></div></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".resolution\">resolution: </span><span class=\"field-value\">-1.0</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".comments\">comments: </span><span class=\"field-value\">no comments</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".description\">description: </span><span class=\"field-value\">Position (x, y) for the first tracking LED.</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".conversion\">conversion: </span><span class=\"field-value\">0.01</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".offset\">offset: </span><span class=\"field-value\">0.0</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".unit\">unit: </span><span class=\"field-value\">meters</span></div><details><summary style=\"display: list-item; margin-left: 0px;\" class=\"container-fields field-key\" title=\".data\"><b>data</b></summary></details><details><summary style=\"display: list-item; margin-left: 0px;\" class=\"container-fields field-key\" title=\".timestamps\"><b>timestamps</b></summary></details><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".timestamps_unit\">timestamps_unit: </span><span class=\"field-value\">seconds</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".interval\">interval: </span><span class=\"field-value\">1</span></div><div style=\"margin-left: 0px;\" class=\"container-fields\"><span class=\"field-key\" title=\".reference_frame\">reference_frame: </span><span class=\"field-value\">(0,0) is not known.</span></div></div>"
],
"text/plain": [
"SpatialSeriesLED1 pynwb.behavior.SpatialSeries at 0x6058780640\n",
"SpatialSeriesLED1 pynwb.behavior.SpatialSeries at 0x6001041792\n",
"Fields:\n",
" comments: no comments\n",
" conversion: 0.01\n",
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"</table><p>... and 4 more rows.</p></details></div>"
],
"text/plain": [
"units pynwb.misc.Units at 0x6062849200\n",
"units pynwb.misc.Units at 0x6002657904\n",
"Fields:\n",
" colnames: ['unit_name' 'spike_times' 'histology' 'hemisphere' 'depth']\n",
" columns: (\n",
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"id": "1eb0cc7d-87cb-4919-a4d7-b7478614477e",
"metadata": {},
"source": [
"[Pynapple](https://pynapple-org.github.io/pynapple/) is a light-weight python library for neurophysiological data analysis that accepts NWB files as input."
"[Pynapple](https://pynapple-org.github.io/pynapple/) is a light-weight python library for neurophysiological data analysis that accepts NWB files as input. Let's install pynapple and a plotting package, seaborn, using `pip`."
]
},
{
"cell_type": "code",
"execution_count": 20,
"id": "e851df97-6b83-42ed-a074-e41113c251e8",
"metadata": {},
"outputs": [
{
"name": "stdout",
"output_type": "stream",
"text": [
"Requirement already satisfied: pynapple in /Users/smprince/anaconda3/envs/bcm-workshop/lib/python3.12/site-packages (0.6.2)\n",
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]
}
],
"source": [
"!pip install pynapple seaborn"
]
},
{
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},
{
"cell_type": "code",
"execution_count": 20,
"execution_count": 21,
"id": "61cd613d-db4d-4608-b71f-2d97a37fb802",
"metadata": {},
"outputs": [
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"┕━━━━━━━━━━━━━━━━━━━━━┷━━━━━━━━━━┙"
]
},
"execution_count": 20,
"execution_count": 21,
"metadata": {},
"output_type": "execute_result"
}
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},
{
"cell_type": "code",
"execution_count": 21,
"execution_count": 22,
"id": "b4957059-c4c0-4c53-99c9-e1855c91d665",
"metadata": {},
"outputs": [
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" 7 1.47 t4c1 MEC LII 0.0024"
]
},
"execution_count": 21,
"execution_count": 22,
"metadata": {},
"output_type": "execute_result"
}
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},
{
"cell_type": "code",
"execution_count": 22,
"execution_count": 23,
"id": "4e24caa5-a4f7-4b2e-87a7-aa085295ae41",
"metadata": {},
"outputs": [
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"dtype: float64, shape: (30000, 2)"
]
},
"execution_count": 22,
"execution_count": 23,
"metadata": {},
"output_type": "execute_result"
}
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},
{
"cell_type": "code",
"execution_count": 23,
"execution_count": 24,
"id": "ff2fedad-370e-4bca-a000-6268d8fca444",
"metadata": {},
"outputs": [
{
"name": "stderr",
"output_type": "stream",
"text": [
"/Users/smprince/anaconda3/envs/houston-workshop/lib/python3.12/site-packages/pynapple/process/tuning_curves.py:223: RuntimeWarning: invalid value encountered in divide\n",
"/Users/smprince/anaconda3/envs/bcm-workshop/lib/python3.12/site-packages/pynapple/process/tuning_curves.py:223: RuntimeWarning: invalid value encountered in divide\n",
" count = count / occupancy\n"
]
},
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},
{
"cell_type": "code",
"execution_count": 24,
"execution_count": 25,
"id": "24c52805-0a0a-4354-a60c-cef9ab539728",
"metadata": {},
"outputs": [
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},
{
"cell_type": "code",
"execution_count": 25,
"execution_count": 26,
"id": "ecaa28a4-f9d6-466d-ba76-53e690bfdedc",
"metadata": {},
"outputs": [],
Expand Down

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