4.0.3

• DNAscent is now fully compatible with Dorado and POD5. These are now recommended over Guppy and FAST5.
• DNAscent outputs BrdU and EdU calls in modbam format. This is now recommended over the previous human-readable table of BrdU and EdU probabilities.
• The workflow and visualisation pages of the documentation have been revamped to show how to use these changes.
• Guppy, FAST5, and human-readable detection outputs are all still supported to avoid breaking users’ previous workflows.
• The modbam MM tag for EdU and BrdU specifies N+e? and N+b?, respectively. Users familiar with the SAM optional fields specification will notice that these are actually the one-letter codes for 5fU and 5caU. We have done this as a temporary measure so that BrdU and EdU can be easily shaded and viewed in IGV (View –> Preferences –> Base Mods). We will replace these one-letter codes with the ChEBI IDs for BrdU and EdU as soon as this is supported by IGV.