FOCUS: High throughput processing of the image stacks recorded by electron microscopes
About
A Graphical User Interface designed using C++ and Qt5 with following features:
- An import tool which can continuously monitor the new images
- Scripts which can process these images using in-house and external software. It is easy for the users to define more scripts if required
- A parallel batch queue processor which transfers processing of the scripts to different resources available on the system
- A library which summaries the statistics of the collected images
- A website which can be used to continuously monitor the status of the recorded images
Supported Modes
Focus supports different type of electron microscopy projects which can be set when setting up a new project. Current supported modes are:
- Drift correction only
- Single Particle with drift correction
- 2D Electron Crystallography with drift correction
- Electron tomography with drift correction
List of dependencies and installation instructions: http://lbem-focus.epfl.ch/wiki/doku.php?id=1_0:install-source
Setting up external software tools: http://lbem-focus.epfl.ch/wiki/doku.php?id=1_0:external-tools
General documentation: https://lbem-focus.epfl.ch/wiki/doku.php?id=home
If FOCUS is useful in your work, please cite:
Biyani, N., Righetto, R. D., McLeod, R., Caujolle-Bert, D., Castano-Diez, D., Goldie, K. N., & Stahlberg, H. (2017). FOCUS: The interface between data collection and data processing in cryo-EM. Journal of Structural Biology, 198(2), 124–133. https://doi.org/10.1016/j.jsb.2017.03.007