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NanoJ-Fluidics: open-source fluid exchange in microscopy

NanoJ-Fluidics is an open-source device, composed of easily accessible LEGO-parts, electronics and labware. It is designed to automate and simplify fluid exchange experiments in microscopy. Check the paper in Nature Communications: Automating multimodal microscopy with NanoJ-Fluidics.

It consists of three parts:

• LEGO-based, multiplexable and compact syringe pumps
• A simple "hack" to enable liquid exchange on cell culture dishes
• And a comprehensive electronic and software control suite to control the pumps.

This Wiki provides all the information necessary for researchers to reproduce their own systems and start performing fluidic experiments on their microscopes.

Developers

NanoJ-Fluidics is developed in a collaboration between the Henriques and Leterrier laboratories, with contributions from the community:

• Matthew Meyer (La Jolla Institute of Allergy & Immunology's Microscopy Core): 3D printed syringe pump body, v-slot adaptor, other parts (see section).
• Leo Saunders (University of Colorado Denver): 3D printed syringe pump body (see section).