OMERO Macro Extensions

A plugin for ImageJ to provide macro extensions to access OMERO.

How to install

1. Install the OMERO.insight plugin (if you haven't already).
2. Download the JAR file for this library.
3. Download the JAR file (for this plugin).
4. Place these JAR files in your plugins folder.

How to use

Extensions first have to be loaded:

run("OMERO Extensions");

Connection:

Connecting to OMERO is done using:

Ext.connectToOMERO("host", 4064, "username", "password");

Then, switching group can be performed through:

Ext.switchGroup(groupId);

You can also choose to only list objects for a given user (or all if username is empty or equal to "all"):

Ext.listForUser(username);

When done, you can disconnect using:

Ext.disconnect()

Listing repository objects (projects, datasets, images, tags)

Once connected, projects, datasets, images and tags IDs can be listed, and their name retrieved:

• Projects:

projects = Ext.list("projects");
print(projects);
projectIds = split(projects,",");
projectName = Ext.getName("project", projectIds[0]);
print(projectName);

• Datasets:

datasets = Ext.list("datasets");
print(datasets);
datasetIds = split(datasets,",");
datasetName = Ext.getName("dataset", datasetIds[0]);
print(datasetName);

• Images:

images = Ext.list("images");
print(images);
imageIds = split(images,",");
imageName = Ext.getName("image", imageIds[0]);
print(imageName);

• Screens:

screens = Ext.list("screens");
print(screens);
screenIds = split(screens,",");
screenName = Ext.getName("screen", screenIds[0]);
print(screenName);

• Plates:

plates = Ext.list("plates");
print(plates);
plateIds = split(plates,",");
plateName = Ext.getName("plate", plateIds[0]);
print(plateName);

• Wells:

wells = Ext.list("wells");
print(wells);
wellIds = split(wells,",");
wellName = Ext.getName("well", wellIds[0]);
print(wellName);

• Tags:

tags = Ext.list("tags");
print(tags);
tagIds = split(tags,",");
tagName = Ext.getName("tag", tagIds[0]);
print(tagName);

• Key-Value pairs:

kvpairs = Ext.list("kv-pairs");
print(kvpairs);
kvIds = split(kvpairs,",");
kvName = Ext.getName("kv-pair", kvIds[0]);
print(kvName);

Listing objects with a given name

It is also possible to list objects with a specific name:

• Projects:

projects = Ext.list("projects", "name");

• Datasets:

datasets = Ext.list("datasets", "name");

• Images:

images = Ext.list("images", "name");

• Screens:

screens = Ext.list("screens", "name");

• Plates:

plates = Ext.list("plates", "name");

• Tags:

tags = Ext.list("tags", "name");

Listing objects inside a given repository object

Each object ID can then be used to retrieve the contained objects (IDs and names).

It is, for example, possible to list datasets inside a project:

datasets = Ext.list("datasets", "project", projectIds[0]);
print(datasets);
datasetIds = split(datasets,",");
datasetName = Ext.getName("dataset", datasetIds[0]);
print(datasetName);

It is also possible to list tags attached to an image:

tags = Ext.list("tags", "image", imageIds[0]);
print(tags);
tagIds = split(tags,",");
tagName = Ext.getName("tag", tagIds[0]);
print(tagName);

Key-Value pairs can also be listed for a given object:

pairs = Ext.list("kv-pairs", "image", imageIds[0]);
print(pairs);
pairIds = split(pairs,",");
kvs = Ext.getName("kv-pair", pairIds[0]);
print(kvs);

or:

kvpairs = Ext.getKeyValuePairs("image", imageIds[0]);
print(kvpairs);

or for a given key:

values = Ext.getValues("image", imageIds[0], "key", defaultValue);
print(values);

Similarly, a dataset ID can be used to retrieve its images:

images = Ext.list("images", "dataset", datasetIds[0]);
print(images);
imageIds = split(images,",");
imageName = Ext.getName("image", imageIds[0]);
print(imageName);

Screens, plates and wells can also be browsed this way:

screens = Ext.list("screens");
screenIds = split(screens,",");
screenName = Ext.getName("screen", screenIds[0]);
plates = Ext.list("plates", "screen", screenIds[0]);
plateIds = split(plates,",");
wells = Ext.list("wells", "plate", plateIds[0]);
wellIds = split(wells,",");
images = Ext.list("images", "well", wellIds[0]);
print(images);
imageIds = split(images,",");
imageName = Ext.getName("image", imageIds[0]);
print(imageName);

Finally, intermediate hierarchy levels can be skipped when listing contained objects:

images = Ext.list("images", "project", projectIds[0]);
print(images);
imageIds = split(images,",");
imageName = Ext.getName("image", imageIds[0]);
print(imageName);

Creating projects, datasets, tags and key-value pairs

Projects can be created with Ext.createProject:

projectId = Ext.createProject(name, description);

Datasets can be created with Ext.createDataset:

datasetId = Ext.createDataset(name, description, projectId);

Tags can be created with Ext.createTag:

tagId = Ext.createTag(name, description);

Key-value pairs can be created with Ext.createKeyValuePair:

pairId = Ext.createKeyValuePair(key, value);

Linking/unlinking objects

Objects can be linked with Ext.link, e.g.:

Ext.link("dataset", datasetId, "tag", tagId);

They can also be unlinked with Ext.unlink, e.g.:

Ext.unlink("dataset", datasetId, "kv-pair", pairId);

Deleting objects

Objects can be deleted with Ext.delete:

Ext.delete("project", projectId);

Opening images

Pixel intensities can be retrieved from images:

imageplusID = Ext.getImage(imageIds[0]);

Images can also be cropped on import. In this case, image properties containing the position of the crop in the original image can be retrieved (IMAGE_POS_X, IMAGE_POS_Y, etc.).

This crop can be done by specifying the ROI in one of two ways:

1. using the ROI ID (as a String) from OMERO, for example:

imageplusID = Ext.getImage(imageIds[0], Roi.getProperty("ROI_ID"));

2. as a String of the form: "x:xstart:xend,y:ystart:yend,c:cstart:cend,z:zstart:zend,t:tstart:tend". For example, cropping a 512x512x3x100x5 image starting at (0,0,0,50,3) and ending at (100,100,2,99,3) can be done with:

imageplusID = Ext.getImage(imageIds[0], "x:0:100,y::100,z:50:,t:3");

ROIs from OMERO can also be added to the ROI manager or to the Overlay of the current image (boolean toOverlay). ROIs composed of multiple shapes (eg 3D/4D) will share the same values in the "ROI" and "ROI_ID" properties in ImageJ. These can be optionally changed with the "property" parameter: local indices will be in "property" while OMERO IDs will be in "property + _ID". This is achieved through:

nIJROIs = Ext.getROIs(imageIds[0], toOverlay, property);

Conversely, ImageJ ROIs can also be saved to OMERO (the property is used to group ImageJ shapes into a single 3D/4D ROI on OMERO, if the string is empty, "ROI" is used):

nROIS = Ext.saveROIs(imageId, property);

Saving images

The current image can be saved (as a TIF) to a dataset in OMERO:

newImageId = Ext.importImage(datasetId);

Attaching / deleting files

Files can be attached to a project/dataset/image through Ext.addFile:

fileId = Ext.addFile('image', imageId, path);

They can also be deleted with Ext.deleteFile:

Ext.deleteFile(fileId);

Attaching a table

A table can be created/updated using the results with Ext.addToTable:

Ext.addToTable(tableName, resultsName, imageId, roiProperty);

If a column named ROI containing ROI IDs is present, these will be added to the table. Alternatively, if ROIs that were saved to OMERO are in the ROI Manager and if their name appears in the labels or is in a column named ROI, they will be added too.

The table can then be saved to a project/dataset/image through Ext.saveTable:

Ext.saveTable(tableName, 'dataset', datasetId);

It can then be saved to a delimited text file through Ext.saveTableAsFile (default separator is ','):

Ext.saveTableAsFile(tableName, pathToFile, delimiter);

Work as another user (sudo)

If a user has sudo rights, it is possible to do all the above as another user:

Ext.sudo(otherUsername);

To switch back to the original user, this command should be used:

Ext.endSudo();

License

GPLv2+