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Fixed numbers
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MarvinDo authored Jul 5, 2024
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Expand Up @@ -50,7 +50,7 @@ sudo apt install genometools
sudo apt install libdb-dev
sudo apt-get install libpq-dev libffi-dev libsqlite3-dev
```
### 3. install tools
### 2. install tools
Next, install the following tools: python, venv, htslib, keycloak, redis, vep, ngs_bits, samtools and herediclass. You may choose to install every tool on its own (eg. ```make python```) or install all at once using
```
make tools
Expand All @@ -63,7 +63,7 @@ Again, they can be installed on its own (eg. ```make bootstrap```) or all togeth
```
make js_libs
```
### 4. download annotation databases
### 3. download annotation databases
This step takes the longest time since a lot of data needs to be downloaded and prepared to be digestable by HerediVar.

First install the reference genomes: grch37, grch38 and chainfile or install all at once using
Expand All @@ -83,7 +83,7 @@ make annotation_data

Note: Although HerediVar only annotates the variant identifier from COSMIC it requires a license. Once you have the data you can take a look two conversion scripts that were used: [https://github.com/imgag/megSAP/blob/master/src/Tools/db_converter_cosmic.php](https://github.com/imgag/megSAP/blob/master/src/Tools/db_converter_cosmic.php) and [https://github.com/GC-HBOC/HerediVar/blob/main/data/script/download_cosmic.sh](https://github.com/GC-HBOC/HerediVar/blob/main/data/script/download_cosmic.sh)

### 5. initialize HerediVar database
### 4. initialize HerediVar database
First connect to the mariadb (or mysql) console and create a new scheme:
```
CREATE DATABASE HerediVar;
Expand Down Expand Up @@ -128,7 +128,7 @@ source HerediVar/.venv/bin/activate
python3 HerediVar/tools/init_db.py
```

### 6 Initialize Keycloak
### 5. Initialize Keycloak
Run the following commands from a terminal and replace "xxx" with a username and **STRONG** password.
```
KEYCLOAK_ADMIN=xxx
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