Skip to content

Commit

Permalink
Set the blast db to refseq mitochondrion by default in the workflow
Browse files Browse the repository at this point in the history
  • Loading branch information
Delphine-L committed Nov 1, 2023
1 parent 2856747 commit e1bdf60
Show file tree
Hide file tree
Showing 2 changed files with 49 additions and 89 deletions.
Original file line number Diff line number Diff line change
Expand Up @@ -4,10 +4,6 @@
class: File
location: https://www.dropbox.com/scl/fi/78taipsjo1e2sd6vzia8q/Scaffolded_assembly.fasta?rlkey=naq41o9fkt2ltlgc2dcjbbyz4&dl=1
filetype: fasta
Mitochondria Database for BLAST:
class: File
location: https://www.dropbox.com/scl/fi/2lsraj3n8qxn2r9agwupw/Mitochondria-Database-for-BLAST.blastdbn?rlkey=7xkhj5wrf6yegt6vnt0tqnm0r&dl=1
filetype: blastdbn
Database for Kraken2: viral2019-03
Include specific intervals - Optional: null
SAK input file - Optional: null
Expand Down
Original file line number Diff line number Diff line change
Expand Up @@ -10,7 +10,7 @@
],
"format-version": "0.1",
"license": "BSD-3-Clause",
"release":"0.1",
"release": "0.1",
"name": "Assembly-decontamination-VGP9",
"steps": {
"0": {
Expand Down Expand Up @@ -68,49 +68,17 @@
"workflow_outputs": []
},
"2": {
"annotation": "Select the database to query to identify mitochondrial contaminants.",
"content_id": null,
"errors": null,
"id": 2,
"input_connections": {},
"inputs": [
{
"description": "Select the database to query to identify mitochondrial contaminants.",
"name": "Mitochondria Database for BLAST"
}
],
"label": "Mitochondria Database for BLAST",
"name": "Input dataset",
"outputs": [],
"position": {
"left": 545.00390625,
"top": 675.12109375
},
"tool_id": null,
"tool_state": "{\"optional\": false, \"tag\": null}",
"tool_version": null,
"type": "data_input",
"uuid": "162f7aed-b552-48e4-8ed9-bbf568f8e1a3",
"when": null,
"workflow_outputs": []
},
"3": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1",
"errors": null,
"id": 3,
"id": 2,
"input_connections": {
"infile": {
"id": 0,
"output_name": "output"
}
},
"inputs": [
{
"description": "runtime parameter for tool Text transformation",
"name": "infile"
}
],
"inputs": [],
"label": "lower to uppercase",
"name": "Text transformation",
"outputs": [
Expand Down Expand Up @@ -139,21 +107,21 @@
"owner": "bgruening",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"adv_opts\": {\"adv_opts_selector\": \"basic\", \"__current_case__\": 0}, \"code\": \"/^>/! s/.*/\\\\U&/\", \"infile\": {\"__class__\": \"RuntimeValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}",
"tool_state": "{\"adv_opts\": {\"adv_opts_selector\": \"basic\", \"__current_case__\": 0}, \"code\": \"/^>/! s/.*/\\\\U&/\", \"infile\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}",
"tool_version": "1.1.1",
"type": "tool",
"uuid": "b9b3d9b9-1716-486d-b65c-b0fb196cbc01",
"when": null,
"workflow_outputs": []
},
"4": {
"3": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_dustmasker_wrapper/2.10.1+galaxy2",
"errors": null,
"id": 4,
"id": 3,
"input_connections": {
"db_opts|subject": {
"id": 3,
"id": 2,
"output_name": "output"
}
},
Expand Down Expand Up @@ -185,23 +153,18 @@
"when": null,
"workflow_outputs": []
},
"5": {
"4": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1",
"errors": null,
"id": 5,
"id": 4,
"input_connections": {
"infile": {
"id": 4,
"id": 3,
"output_name": "outfile"
}
},
"inputs": [
{
"description": "runtime parameter for tool Text transformation",
"name": "infile"
}
],
"inputs": [],
"label": "hard-masking",
"name": "Text transformation",
"outputs": [
Expand Down Expand Up @@ -230,25 +193,25 @@
"owner": "bgruening",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"adv_opts\": {\"adv_opts_selector\": \"basic\", \"__current_case__\": 0}, \"code\": \"/^>/!s/[a-z]/N/g\", \"infile\": {\"__class__\": \"RuntimeValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}",
"tool_state": "{\"adv_opts\": {\"adv_opts_selector\": \"basic\", \"__current_case__\": 0}, \"code\": \"s/[a-z]/N/g\", \"infile\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}",
"tool_version": "1.1.1",
"type": "tool",
"uuid": "5089350b-c1a9-498a-9786-9864d484f77a",
"when": null,
"workflow_outputs": []
},
"6": {
"5": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/kraken2/kraken2/2.1.1+galaxy1",
"errors": null,
"id": 6,
"id": 5,
"input_connections": {
"kraken2_database": {
"id": 1,
"output_name": "output"
},
"single_paired|input_sequences": {
"id": 5,
"id": 4,
"output_name": "output"
}
},
Expand Down Expand Up @@ -300,22 +263,23 @@
}
]
},
"7": {
"6": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/devteam/ncbi_blast_plus/ncbi_blastn_wrapper/2.10.1+galaxy2",
"errors": null,
"id": 7,
"id": 6,
"input_connections": {
"db_opts|histdb": {
"id": 2,
"output_name": "output"
},
"query": {
"id": 5,
"id": 4,
"output_name": "output"
}
},
"inputs": [],
"inputs": [
{
"description": "runtime parameter for tool NCBI BLAST+ blastn",
"name": "query"
}
],
"label": "blast mitochondria DB",
"name": "NCBI BLAST+ blastn",
"outputs": [
Expand All @@ -336,21 +300,21 @@
"owner": "devteam",
"tool_shed": "toolshed.g2.bx.psu.edu"
},
"tool_state": "{\"adv_opts\": {\"adv_opts_selector\": \"basic\", \"__current_case__\": 0}, \"blast_type\": \"blastn\", \"db_opts\": {\"db_opts_selector\": \"histdb\", \"__current_case__\": 1, \"database\": \"\", \"histdb\": {\"__class__\": \"ConnectedValue\"}, \"subject\": \"\"}, \"evalue_cutoff\": \"0.001\", \"output\": {\"out_format\": \"cols\", \"__current_case__\": 2, \"std_cols\": [\"qseqid\", \"sseqid\", \"length\", \"qstart\", \"qend\", \"evalue\"], \"ext_cols\": [\"qlen\"], \"ids_cols\": null, \"misc_cols\": [\"qcovs\", \"qcovhsp\"], \"tax_cols\": null}, \"query\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}",
"tool_state": "{\"adv_opts\": {\"adv_opts_selector\": \"basic\", \"__current_case__\": 0}, \"blast_type\": \"blastn\", \"db_opts\": {\"db_opts_selector\": \"db\", \"__current_case__\": 0, \"database\": \"13apr2014-htgs\", \"histdb\": \"\", \"subject\": \"\"}, \"evalue_cutoff\": \"0.001\", \"output\": {\"out_format\": \"cols\", \"__current_case__\": 2, \"std_cols\": [\"qseqid\", \"sseqid\", \"length\", \"qstart\", \"qend\", \"evalue\"], \"ext_cols\": [\"qlen\"], \"ids_cols\": null, \"misc_cols\": [\"qcovs\", \"qcovhsp\"], \"tax_cols\": null}, \"query\": {\"__class__\": \"RuntimeValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}",
"tool_version": "2.10.1+galaxy2",
"type": "tool",
"uuid": "1737bad0-48d9-489e-9a45-d6f3d3fcb900",
"when": null,
"workflow_outputs": []
},
"8": {
"7": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_grep_tool/1.1.1",
"errors": null,
"id": 8,
"id": 7,
"input_connections": {
"infile": {
"id": 6,
"id": 5,
"output_name": "classified_out_s"
}
},
Expand Down Expand Up @@ -382,14 +346,14 @@
"when": null,
"workflow_outputs": []
},
"9": {
"8": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/iuc/parse_mito_blast/parse_mito_blast/1.0.2+galaxy0",
"errors": null,
"id": 9,
"id": 8,
"input_connections": {
"blastout": {
"id": 7,
"id": 6,
"output_name": "output1"
}
},
Expand Down Expand Up @@ -425,14 +389,14 @@
"when": null,
"workflow_outputs": []
},
"10": {
"9": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1",
"errors": null,
"id": 10,
"id": 9,
"input_connections": {
"infile": {
"id": 8,
"id": 7,
"output_name": "output"
}
},
Expand Down Expand Up @@ -472,14 +436,14 @@
"when": null,
"workflow_outputs": []
},
"11": {
"10": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_sed_tool/1.1.1",
"errors": null,
"id": 11,
"id": 10,
"input_connections": {
"infile": {
"id": 9,
"id": 8,
"output_name": "mito_scaff_names"
}
},
Expand Down Expand Up @@ -511,14 +475,14 @@
"when": null,
"workflow_outputs": []
},
"12": {
"11": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_find_and_replace/1.1.4",
"errors": null,
"id": 12,
"id": 11,
"input_connections": {
"infile": {
"id": 10,
"id": 9,
"output_name": "output"
}
},
Expand Down Expand Up @@ -550,19 +514,19 @@
"when": null,
"workflow_outputs": []
},
"13": {
"12": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/text_processing/tp_cat/0.1.1",
"errors": null,
"id": 13,
"id": 12,
"input_connections": {
"inputs": [
{
"id": 11,
"id": 10,
"output_name": "output"
},
{
"id": 12,
"id": 11,
"output_name": "outfile"
}
]
Expand Down Expand Up @@ -601,18 +565,18 @@
}
]
},
"14": {
"13": {
"annotation": "",
"content_id": "toolshed.g2.bx.psu.edu/repos/bgruening/gfastats/gfastats/1.3.6+galaxy0",
"errors": null,
"id": 14,
"id": 13,
"input_connections": {
"input_file": {
"id": 0,
"output_name": "output"
},
"target_condition|exclude_bed": {
"id": 13,
"id": 12,
"output_name": "out_file1"
}
},
Expand Down Expand Up @@ -663,6 +627,6 @@
"tags": [
"VGP_curated"
],
"uuid": "2aeb8e6f-0fe3-445e-b540-5011d063a600",
"version": 9
"uuid": "742bb06f-5b88-4b4c-8096-28c9fe4350d6",
"version": 13
}

0 comments on commit e1bdf60

Please sign in to comment.