create_tiltseries.py

Script that creates simulated electron tomography tilt series using from SBEM MRC using ET-SPEC from the commandline.

ET-SPEC is comprised of two parts, a graphical user interface and a set of command line tools that do the actual work. This create_tiltseries.py utilizes the ET-SPEC command line tools two create simulated electron tomography tilt series.

To see all the options for create_tiltseries.py run the following:

create_tiltseries.py --help

Creating single tilt series example

The following assumes foo.mrc is a SBEM MRC file containing say 30 slices. The command below utilizes all defaults so a single tilt series where the sample is tilted from -60 degrees to 60 degrees in 2 degree increments is run.

create_tiltseries.py --etspecbin <path to etspec bin dir> foo.mrc resultdir