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MAGeCK fails when counts matrix has an empty geneID #34

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slsevilla opened this issue Nov 20, 2023 · 0 comments
Open

MAGeCK fails when counts matrix has an empty geneID #34

slsevilla opened this issue Nov 20, 2023 · 0 comments
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slsevilla commented Nov 20, 2023

Description of the bug

During mageck test pipeline is failing

 mageck test \
    -k cruise_1.count.txt \
    -t D8_KO_Dabrafenib_R1,D8_KO_Dabrafenib_R2,D8_KO_Dabrafenib_R3,D8_KO_Dabrafenib_R4 \
    -c D8_KO_DMSO_R1,D8_KO_DMSO_R2,D8_KO_DMSO_R3,D8_KO_DMSO_R4 \
    -n cruise_1
ERROR @ Mon, 20 Nov 2023 00:04:45: Error: empty gene ID is not allowed at line 268. Please double-check your read count table file. 

When reviewing the contrast table cruise_1.count.txt this line has no geneID.

Proposed solution

Add in a check for the library that each row has three columns

@slsevilla slsevilla added the bug Something isn't working label Nov 20, 2023
@slsevilla slsevilla self-assigned this Nov 20, 2023
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