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tclose committed Dec 20, 2023
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from pydra import Workflow
from pydra.engine.specs import File
from pydra.tasks.mrtrix3.v3_0 import (
fivett2vis,
fivettgen_hsvs,
labelconvert,
labelsgmfix,
)
# from pydra.tasks.mrtrix3.v3_0 import (
# fivett2vis,
# fivettgen_hsvs,
# labelconvert,
# labelsgmfix,
# )
from fileformats.medimage import NiftiGz, MghGz
from fileformats.medimage_mrtrix3 import ImageFormat as MIF
from pydra.tasks.fastsurfer.latest import fastsurfer
# from pydra.tasks.fastsurfer.latest import fastsurfer


def t1_preproc():
# Define the path and output_path variables
# path = '/Users/arkievdsouza/Documents/NIFdata/ds000114'
output_path = "/Users/arkievdsouza/git/t1-pipeline/working-dir"

# Define the input_spec for the workflow
input_spec = {
"t1w": NiftiGz,
"fs_license": File,
"sub_ID": str,
"default_file": File,
"freesurfer_LUT": File,
"segmentation": MghGz,
}
output_spec = {"fTT_image": MIF, "vis_image": MIF, "parc_image": MIF}

# Create a workflow
wf = Workflow(
name="T1preproc_workflow",
input_spec=input_spec,
cache_dir=output_path,
output_spec=output_spec,
)

# FastSurfer Task
wf.add(
fastsurfer(
T1_files=wf.lzin.t1w,
fs_license=wf.lzin.fs_license,
subject_id=wf.lzin.sub_ID,
threads=7,
parallel=True,
name="FastSurfer_task",
py="python3.11",
norm_img="norm.nii.gz",
aparcaseg="aparcaseg.nii.gz",
surf_only=True,
seg=wf.lzin.segmentation,
)
)

# #################################################
# # Five Tissue Type Generation and visualisation #
# #################################################

# Five tissue-type task
wf.add(
fivettgen_hsvs(
input=wf.FastSurfer_task.lzout.subjects_dir,
output="fTT_hsvs.mif",
name="fTTgen_task",
nocrop=True,
nocleanup=True,
white_stem=True,
)
)

# Five tissue-type visualisation task
wf.add(
fivett2vis(
input=wf.fTTgen_task.lzout.output.cast(MIF),
output="fTT_hsvs_vis.mif",
name="fTTvis_task",
)
)

# ###################
# # DK parcellation #
# ###################

# relabel segmenetation to integers indexed from 1 to 84
wf.add(
labelconvert(
path_in=wf.FastSurfer_task.lzout.aparcaseg_img,
lut_in=wf.lzin.freesurfer_LUT,
lut_out=wf.lzin.default_file,
path_out="nodes.mif",
name="LabelConvert_task",
)
)

# # Replace FreeSurfer’s estimates of sub-cortical grey matter structures with estimates from FSL’s FIRST tool
wf.add(
labelsgmfix(
parc=wf.LabelConvert_task.lzout.path_out,
t1=wf.FastSurfer_task.lzout.norm_img,
lut=wf.lzin.default_file,
output="parcellation_image_DK_unregistered.mif",
name="SGMfix_task",
nocleanup=True,
premasked=True,
sgm_amyg_hipp=True,
)
)

##################
# WORKFLOW SETUP #
##################

wf.set_output(("fTT_image", wf.fTTgen_task.lzout.output.cast(MIF)))
wf.set_output(("vis_image", wf.fTTvis_task.lzout.output.cast(MIF)))
wf.set_output(("parc_image", wf.SGMfix_task.lzout.output.cast(MIF)))

# ## Execute the workflow (FastSurfer, NIF data)
# # Define the input_spec for the workflow
# input_spec = {
# "t1w": NiftiGz,
# "fs_license": File,
# "sub_ID": str,
# "default_file": File,
# "freesurfer_LUT": File,
# "segmentation": MghGz,
# }
# output_spec = {"fTT_image": MIF, "vis_image": MIF, "parc_image": MIF}

# # Create a workflow
# wf = Workflow(
# name="T1preproc_workflow",
# input_spec=input_spec,
# cache_dir=output_path,
# output_spec=output_spec,
# )

# # FastSurfer Task
# wf.add(
# fastsurfer(
# T1_files=wf.lzin.t1w,
# fs_license=wf.lzin.fs_license,
# subject_id=wf.lzin.sub_ID,
# threads=7,
# parallel=True,
# name="FastSurfer_task",
# py="python3.11",
# norm_img="norm.nii.gz",
# aparcaseg="aparcaseg.nii.gz",
# surf_only=True,
# seg=wf.lzin.segmentation,
# )
# )

# # #################################################
# # # Five Tissue Type Generation and visualisation #
# # #################################################

# # Five tissue-type task
# wf.add(
# fivettgen_hsvs(
# input=wf.FastSurfer_task.lzout.subjects_dir,
# output="fTT_hsvs.mif",
# name="fTTgen_task",
# nocrop=True,
# nocleanup=True,
# white_stem=True,
# )
# )

# # Five tissue-type visualisation task
# wf.add(
# fivett2vis(
# input=wf.fTTgen_task.lzout.output.cast(MIF),
# output="fTT_hsvs_vis.mif",
# name="fTTvis_task",
# )
# )

# # ###################
# # # DK parcellation #
# # ###################

# # relabel segmenetation to integers indexed from 1 to 84
# wf.add(
# labelconvert(
# path_in=wf.FastSurfer_task.lzout.aparcaseg_img,
# lut_in=wf.lzin.freesurfer_LUT,
# lut_out=wf.lzin.default_file,
# path_out="nodes.mif",
# name="LabelConvert_task",
# )
# )

# # # Replace FreeSurfer’s estimates of sub-cortical grey matter structures with estimates from FSL’s FIRST tool
# wf.add(
# labelsgmfix(
# parc=wf.LabelConvert_task.lzout.path_out,
# t1=wf.FastSurfer_task.lzout.norm_img,
# lut=wf.lzin.default_file,
# output="parcellation_image_DK_unregistered.mif",
# name="SGMfix_task",
# nocleanup=True,
# premasked=True,
# sgm_amyg_hipp=True,
# )
# )

# ##################
# # WORKFLOW SETUP #
# ##################

# wf.set_output(("fTT_image", wf.fTTgen_task.lzout.output.cast(MIF)))
# wf.set_output(("vis_image", wf.fTTvis_task.lzout.output.cast(MIF)))
# wf.set_output(("parc_image", wf.SGMfix_task.lzout.output.cast(MIF)))

# # ## Execute the workflow (FastSurfer, NIF data)
# # result = wf(
# # t1w="/Users/arkievdsouza/Documents/NIFdata/ds000114/sub-01/ses-retest/anat/sub-01_ses-retest_T1w.nii.gz",
# # fs_license="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FS_license.txt",
# # sub_ID="sub-01_ses-retest",
# # plugin="serial"
# # )

# # Execute the workflow (FastSurfer, HCP data)
# result = wf(
# t1w="/Users/arkievdsouza/Documents/NIFdata/ds000114/sub-01/ses-retest/anat/sub-01_ses-retest_T1w.nii.gz",
# t1w="/Users/arkievdsouza/Documents/100307/100307_FastSurfer/mri/orig.nii.gz",
# fs_license="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FS_license.txt",
# sub_ID="sub-01_ses-retest",
# plugin="serial"
# sub_ID="100307",
# default_file="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/fs_default.txt",
# freesurfer_LUT="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FreeSurferColorLUT.txt",
# segmentation="/Users/arkievdsouza/git/t1-pipeline/working-dir/fastsurfer_0425d50a2d1bdc642ef8feb235ec3855/subjects_dir/100307/mri/aparc.DKTatlas+aseg.deep.mgz",
# plugin="serial",
# )

# Execute the workflow (FastSurfer, HCP data)
result = wf(
t1w="/Users/arkievdsouza/Documents/100307/100307_FastSurfer/mri/orig.nii.gz",
fs_license="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FS_license.txt",
sub_ID="100307",
default_file="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/fs_default.txt",
freesurfer_LUT="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FreeSurferColorLUT.txt",
segmentation="/Users/arkievdsouza/git/t1-pipeline/working-dir/fastsurfer_0425d50a2d1bdc642ef8feb235ec3855/subjects_dir/100307/mri/aparc.DKTatlas+aseg.deep.mgz",
plugin="serial",
)

# print(f"Processed output generated at '{result.output.dwi_preproc}'")

# create_dotfile

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