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...ydneyimaging/australianimagingservice/community/au/edu/sydney/sydneyimaging/t1_preproc.py
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from pydra import Workflow | ||
from pydra.engine.specs import File | ||
from pydra.tasks.mrtrix3.v3_0 import ( | ||
fivett2vis, | ||
fivettgen_hsvs, | ||
labelconvert, | ||
labelsgmfix, | ||
) | ||
# from pydra.tasks.mrtrix3.v3_0 import ( | ||
# fivett2vis, | ||
# fivettgen_hsvs, | ||
# labelconvert, | ||
# labelsgmfix, | ||
# ) | ||
from fileformats.medimage import NiftiGz, MghGz | ||
from fileformats.medimage_mrtrix3 import ImageFormat as MIF | ||
from pydra.tasks.fastsurfer.latest import fastsurfer | ||
# from pydra.tasks.fastsurfer.latest import fastsurfer | ||
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def t1_preproc(): | ||
# Define the path and output_path variables | ||
# path = '/Users/arkievdsouza/Documents/NIFdata/ds000114' | ||
output_path = "/Users/arkievdsouza/git/t1-pipeline/working-dir" | ||
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# Define the input_spec for the workflow | ||
input_spec = { | ||
"t1w": NiftiGz, | ||
"fs_license": File, | ||
"sub_ID": str, | ||
"default_file": File, | ||
"freesurfer_LUT": File, | ||
"segmentation": MghGz, | ||
} | ||
output_spec = {"fTT_image": MIF, "vis_image": MIF, "parc_image": MIF} | ||
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# Create a workflow | ||
wf = Workflow( | ||
name="T1preproc_workflow", | ||
input_spec=input_spec, | ||
cache_dir=output_path, | ||
output_spec=output_spec, | ||
) | ||
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# FastSurfer Task | ||
wf.add( | ||
fastsurfer( | ||
T1_files=wf.lzin.t1w, | ||
fs_license=wf.lzin.fs_license, | ||
subject_id=wf.lzin.sub_ID, | ||
threads=7, | ||
parallel=True, | ||
name="FastSurfer_task", | ||
py="python3.11", | ||
norm_img="norm.nii.gz", | ||
aparcaseg="aparcaseg.nii.gz", | ||
surf_only=True, | ||
seg=wf.lzin.segmentation, | ||
) | ||
) | ||
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# ################################################# | ||
# # Five Tissue Type Generation and visualisation # | ||
# ################################################# | ||
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# Five tissue-type task | ||
wf.add( | ||
fivettgen_hsvs( | ||
input=wf.FastSurfer_task.lzout.subjects_dir, | ||
output="fTT_hsvs.mif", | ||
name="fTTgen_task", | ||
nocrop=True, | ||
nocleanup=True, | ||
white_stem=True, | ||
) | ||
) | ||
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# Five tissue-type visualisation task | ||
wf.add( | ||
fivett2vis( | ||
input=wf.fTTgen_task.lzout.output.cast(MIF), | ||
output="fTT_hsvs_vis.mif", | ||
name="fTTvis_task", | ||
) | ||
) | ||
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# ################### | ||
# # DK parcellation # | ||
# ################### | ||
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# relabel segmenetation to integers indexed from 1 to 84 | ||
wf.add( | ||
labelconvert( | ||
path_in=wf.FastSurfer_task.lzout.aparcaseg_img, | ||
lut_in=wf.lzin.freesurfer_LUT, | ||
lut_out=wf.lzin.default_file, | ||
path_out="nodes.mif", | ||
name="LabelConvert_task", | ||
) | ||
) | ||
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# # Replace FreeSurfer’s estimates of sub-cortical grey matter structures with estimates from FSL’s FIRST tool | ||
wf.add( | ||
labelsgmfix( | ||
parc=wf.LabelConvert_task.lzout.path_out, | ||
t1=wf.FastSurfer_task.lzout.norm_img, | ||
lut=wf.lzin.default_file, | ||
output="parcellation_image_DK_unregistered.mif", | ||
name="SGMfix_task", | ||
nocleanup=True, | ||
premasked=True, | ||
sgm_amyg_hipp=True, | ||
) | ||
) | ||
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################## | ||
# WORKFLOW SETUP # | ||
################## | ||
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wf.set_output(("fTT_image", wf.fTTgen_task.lzout.output.cast(MIF))) | ||
wf.set_output(("vis_image", wf.fTTvis_task.lzout.output.cast(MIF))) | ||
wf.set_output(("parc_image", wf.SGMfix_task.lzout.output.cast(MIF))) | ||
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# ## Execute the workflow (FastSurfer, NIF data) | ||
# # Define the input_spec for the workflow | ||
# input_spec = { | ||
# "t1w": NiftiGz, | ||
# "fs_license": File, | ||
# "sub_ID": str, | ||
# "default_file": File, | ||
# "freesurfer_LUT": File, | ||
# "segmentation": MghGz, | ||
# } | ||
# output_spec = {"fTT_image": MIF, "vis_image": MIF, "parc_image": MIF} | ||
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# # Create a workflow | ||
# wf = Workflow( | ||
# name="T1preproc_workflow", | ||
# input_spec=input_spec, | ||
# cache_dir=output_path, | ||
# output_spec=output_spec, | ||
# ) | ||
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# # FastSurfer Task | ||
# wf.add( | ||
# fastsurfer( | ||
# T1_files=wf.lzin.t1w, | ||
# fs_license=wf.lzin.fs_license, | ||
# subject_id=wf.lzin.sub_ID, | ||
# threads=7, | ||
# parallel=True, | ||
# name="FastSurfer_task", | ||
# py="python3.11", | ||
# norm_img="norm.nii.gz", | ||
# aparcaseg="aparcaseg.nii.gz", | ||
# surf_only=True, | ||
# seg=wf.lzin.segmentation, | ||
# ) | ||
# ) | ||
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# # ################################################# | ||
# # # Five Tissue Type Generation and visualisation # | ||
# # ################################################# | ||
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# # Five tissue-type task | ||
# wf.add( | ||
# fivettgen_hsvs( | ||
# input=wf.FastSurfer_task.lzout.subjects_dir, | ||
# output="fTT_hsvs.mif", | ||
# name="fTTgen_task", | ||
# nocrop=True, | ||
# nocleanup=True, | ||
# white_stem=True, | ||
# ) | ||
# ) | ||
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# # Five tissue-type visualisation task | ||
# wf.add( | ||
# fivett2vis( | ||
# input=wf.fTTgen_task.lzout.output.cast(MIF), | ||
# output="fTT_hsvs_vis.mif", | ||
# name="fTTvis_task", | ||
# ) | ||
# ) | ||
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# # ################### | ||
# # # DK parcellation # | ||
# # ################### | ||
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# # relabel segmenetation to integers indexed from 1 to 84 | ||
# wf.add( | ||
# labelconvert( | ||
# path_in=wf.FastSurfer_task.lzout.aparcaseg_img, | ||
# lut_in=wf.lzin.freesurfer_LUT, | ||
# lut_out=wf.lzin.default_file, | ||
# path_out="nodes.mif", | ||
# name="LabelConvert_task", | ||
# ) | ||
# ) | ||
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# # # Replace FreeSurfer’s estimates of sub-cortical grey matter structures with estimates from FSL’s FIRST tool | ||
# wf.add( | ||
# labelsgmfix( | ||
# parc=wf.LabelConvert_task.lzout.path_out, | ||
# t1=wf.FastSurfer_task.lzout.norm_img, | ||
# lut=wf.lzin.default_file, | ||
# output="parcellation_image_DK_unregistered.mif", | ||
# name="SGMfix_task", | ||
# nocleanup=True, | ||
# premasked=True, | ||
# sgm_amyg_hipp=True, | ||
# ) | ||
# ) | ||
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# ################## | ||
# # WORKFLOW SETUP # | ||
# ################## | ||
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# wf.set_output(("fTT_image", wf.fTTgen_task.lzout.output.cast(MIF))) | ||
# wf.set_output(("vis_image", wf.fTTvis_task.lzout.output.cast(MIF))) | ||
# wf.set_output(("parc_image", wf.SGMfix_task.lzout.output.cast(MIF))) | ||
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# # ## Execute the workflow (FastSurfer, NIF data) | ||
# # result = wf( | ||
# # t1w="/Users/arkievdsouza/Documents/NIFdata/ds000114/sub-01/ses-retest/anat/sub-01_ses-retest_T1w.nii.gz", | ||
# # fs_license="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FS_license.txt", | ||
# # sub_ID="sub-01_ses-retest", | ||
# # plugin="serial" | ||
# # ) | ||
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# # Execute the workflow (FastSurfer, HCP data) | ||
# result = wf( | ||
# t1w="/Users/arkievdsouza/Documents/NIFdata/ds000114/sub-01/ses-retest/anat/sub-01_ses-retest_T1w.nii.gz", | ||
# t1w="/Users/arkievdsouza/Documents/100307/100307_FastSurfer/mri/orig.nii.gz", | ||
# fs_license="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FS_license.txt", | ||
# sub_ID="sub-01_ses-retest", | ||
# plugin="serial" | ||
# sub_ID="100307", | ||
# default_file="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/fs_default.txt", | ||
# freesurfer_LUT="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FreeSurferColorLUT.txt", | ||
# segmentation="/Users/arkievdsouza/git/t1-pipeline/working-dir/fastsurfer_0425d50a2d1bdc642ef8feb235ec3855/subjects_dir/100307/mri/aparc.DKTatlas+aseg.deep.mgz", | ||
# plugin="serial", | ||
# ) | ||
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# Execute the workflow (FastSurfer, HCP data) | ||
result = wf( | ||
t1w="/Users/arkievdsouza/Documents/100307/100307_FastSurfer/mri/orig.nii.gz", | ||
fs_license="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FS_license.txt", | ||
sub_ID="100307", | ||
default_file="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/fs_default.txt", | ||
freesurfer_LUT="/Users/arkievdsouza/Desktop/FastSurferTesting/ReferenceFiles/FreeSurferColorLUT.txt", | ||
segmentation="/Users/arkievdsouza/git/t1-pipeline/working-dir/fastsurfer_0425d50a2d1bdc642ef8feb235ec3855/subjects_dir/100307/mri/aparc.DKTatlas+aseg.deep.mgz", | ||
plugin="serial", | ||
) | ||
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# print(f"Processed output generated at '{result.output.dwi_preproc}'") | ||
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# create_dotfile |