No obviously periodicity are detected from bam file when running metaplots step

[DooYal]

When I process metaplots, the log printed that:

No obviously periodicity are detected from bam file SRR22462570/SRR22462570.Aligned.toTranscriptome.out.bam,
it could be due to poor quality sequencing.
Please check the metagene plots and try again by lowering the value of frame0_percent

Even thought used the following parameters
metaplots -a $genecode.v44.ribo.anno -r $sample.Aligned.toTranscriptome.out.bam -o $sample.ribocode -f0_percent 0.01
The log still reported the same contents, which causes RiboCode to interrput

[liuliuliu0304]

I got the same trouble

[Saurabh-plantist]

I am wondering if you heard anything from the developer about this message or figure out the solution.

Best

[DooYal]

I am wondering if you heard anything from the developer about this message or figure out the solution.

Best

metaplots -a gencode.ribo.anno -r $sample.Aligned.toTranscriptome.out.bam -o $sample.ribocode -pv1 1 -pv2 1, the higher p value cut-offs possibly output some results