From 25cc1e67bc38df286306eed477dbd364b6a8dee3 Mon Sep 17 00:00:00 2001 From: wyang17 Date: Wed, 16 May 2018 06:42:41 -0400 Subject: [PATCH] Updated README --- README.md | 22 +++++----------------- 1 file changed, 5 insertions(+), 17 deletions(-) diff --git a/README.md b/README.md index d094da3..9e98414 100644 --- a/README.md +++ b/README.md @@ -73,23 +73,11 @@ _*If installing these software with conda is unsuccessful, we recommend installi -## Pipeline ## - -#### Inputs #### -The pipeline requires the following inputs: -- BEDfile of Transposable Elements of interest -- STAR index of genome build of interest - -If you have trouble obtaining these items on your own, you can use the steps fetch_ to download the chromosome fasta files, Repeatmasker file and create a STAR index with your transposable elements of interest. - - - - #### Pipeline Steps #### -Preparation +Preparation Stage 1) [Fetch:](#squire-fetch) Downloads input files from RefGene and generates STAR index Only needs to be done once initially to acquire genomic input files or if a new build is desired. @@ -97,7 +85,7 @@ Preparation 2) [Clean:](#squire-clean) Filters Repeatmasker file for Repeats of interest, collapses overlapping repeats, and returns as BED file. -Quantification +Quantification Stage 1) [Map:](#squire-map) Aligns RNAseq data @@ -106,12 +94,12 @@ Quantification Quantifies RNAseq reads aligning to TEs -Analysis +Analysis Stage 1) [Call:](#squire-call) Compiles and outputs differential expression from multiple alignments -Follow-up +Follow-up Stage 1) [Draw:](#squire-draw) Creates BEDgraphs from RNAseq data @@ -389,4 +377,4 @@ INSTRUCTIONS * `qsub -cwd call.sh arguments.sh` * `qsub -cwd loop_draw.sh arguments.sh` -5. If a memory or segmentation fault error occurs, edit the `#$ -l mem_free` and `#$ -l h_vmem` lines to increase memory usage for the appropriate script. \ No newline at end of file +5. If a memory or segmentation fault error occurs, edit the `#$ -l mem_free` and `#$ -l h_vmem` lines to increase memory usage for the appropriate script.