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illumination.html
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<!DOCTYPE html>
<html>
<head>
<meta charset="utf-8">
<title>Strobe-enhanced microscopy stage</title>
<meta http-equiv="X-UA-Compatible" content="IE=edge">
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<body>
<header class="site-header">
<div class="wrapper header-wrapper" >
<div class=header-text>
<h1 class="site-title">Strobe-enhanced microscopy stage</h1>
<p class="author">by Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="affiliation">Latin American Hub for Bioimaging Through Open Hardware (LIBRE hub)</p>
</div>
</div>
</header>
<div class="page-content">
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<ul class="nav-list">
<li>
<a class="navhome" href="./">
Strobe-enhanced microscopy stage
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<a class="active" href="./3-level-station.html">
Build the 3-level microscopy stage
</a>
<ul class="sub-nav-list">
<li>
<a class="not-active" href="./printing.html">
Print the plastic parts
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Laser cut the acrylic parts
</a>
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<a class="not-active" href="./assemble-focus-mechanism.html">
Assemble the focus mechanism
</a>
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<a class="not-active" href="./basic-optics-module.html">
Assemble the basics optics module
</a>
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<a class="not-active" href="./attach-parts-to-top-plate.html">
Attach parts to the top plate
</a>
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<a class="not-active" href="./attach-parts-to-middle-plate.html">
Attach parts to the middle plate
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<a class="not-active" href="./attach-parts-to-bottom-plate.html">
Attach parts to the bottom plate
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<a class="not-active" href="./mount-optics.html">
Mount the optics
</a>
</li>
<li>
<a class="not-active" href="./wiring.html">
Mount the electronics
</a>
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<a class="active" href="./illumination.html">
Assemble the illumination
</a>
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Assembly complete
</a>
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</ul>
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Software Installation
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Use your Stage
</a>
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<a class="not-active" href="./troubleshooting.html">
Troubleshooting
</a>
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<a class="not-active" href="./variants_accesories.html">
Customisation and accessories
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</li>
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</nav>
</div>
<div class="wrapper">
<h1 id="assemble-the-illumination">Assemble the illumination</h1>
<div class="pagebom">
<h3 id="tool">Tools</h3>
<ul>
<li>1 <a Class="bom" href="parts/tools/screwdriver.html">#1 pozidrive screwdriver</a> </li>
<li>1 <a Class="bom" href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm Ball-end Allen key</a> </li>
<li>1 <a Class="bom" href="parts/tools/crimper.html">Crimper</a> </li>
<li>1 <a Class="bom" href="parts/tools/pliers.html">Needle-nose plier</a> </li>
<li>1 <a Class="bom" href="parts/tools/precision-wire-cutters.html">Precision wire cutter</a> </li>
<li>1 <a Class="bom" href="parts/tools/soldiron.html">Soldering iron</a> </li>
</ul>
<h3 id="consumable">Consumables</h3>
<ul>
<li>2 <a Class="bom" href="parts/consumables/gloves.html">Nitrile gloves</a> </li>
</ul>
<h3 id="electronic">Electronic Components</h3>
<ul>
<li>1 <a Class="bom" href="parts/elect/2pin-DuPont-female-housing.html">2 pin Du Pont connector female housing</a> </li>
<li>1 <a Class="bom" href="parts/elect/30x30x10mm-heatsink.html">30x30x10mm Heat sink</a> </li>
<li>2 <a Class="bom" href="parts/elect/protoboard-cables.html">Conductor Flexible Cables</a> </li>
<li>1 <a Class="bom" href="parts/elect/double-sided-adhesive-tape.html">Double-sided self adhesive tape</a> </li>
<li>1 <a Class="bom" href="parts/elect/double-sided-thermal-tape.html">Double-sided thermal tape</a> </li>
<li>1 <a Class="bom" href="parts/elect/high-power-star-LED.html">High-power star LED</a> </li>
<li>2 <a Class="bom" href="parts/elect/male-crimp-pin.html">Male Crimp Pin</a> </li>
</ul>
<h3 id="mechanic">Mechanical Components</h3>
<ul>
<li>1 <a Class="bom" href="parts/mech/Hinsert.html">Heat insert</a> </li>
<li>4 <a Class="bom" href="parts/mech/nuts.html">M3 nut</a> </li>
<li>3 <a Class="bom" href="parts/mech/M3-10.html">M3x10mm cap head screw</a> </li>
<li>1 <a Class="bom" href="parts/mech/X-M3-20.html">M3x20mm pozi pan head screw</a> </li>
</ul>
<h3 id="optical">Optical Components</h3>
<ul>
<li>1 <a Class="bom" href="parts/optics/8.7-20mm-circular-lens.html">Star-LED lens</a> </li>
</ul>
<h3 id="printedpart">Printed Parts</h3>
<ul>
<li>1 <a Class="bom" href="models/arm-pin.html">Hinge pin</a> </li>
<li>1 <a Class="bom" href="models/led-arm.html">Illumination arm</a> </li>
<li>1 <a Class="bom" href="models/illumination-base.html">Illumination base</a> </li>
<li>1 <a Class="bom" href="models/illumination-slider.html">Illumination slider</a> </li>
<li>1 <a Class="bom" href="models/illumination-thumbscrew.html">Illumination thumbscrew</a> </li>
<li>1 <a Class="bom" href="models/led-cover.html">LED cover</a> </li>
</ul>
</div>
<p>In this section, we are assembling the strobe illuminator. This mounts the high-power LED and condenser lens above the sample so the microscope can image the transmitted light.</p>
<h2 class="page-step" id="visually-inspect-the-3d-parts">Step 1: Visually inspect the 3D parts</h2>
<ul>
<li>Verify the slot of <a href="models/illumination-thumbscrew.html">thumbscrew</a> is clean. If strings of plastic are present, use a <a href="parts/tools/pliers.html">needle-nose plier</a> to remove them.</li>
<li>There should be a space between the thinner section and the body of the <a href="models/illumination-slider.html">illumination slider</a>. If not, use a plier to separate them.</li>
</ul>
<h2 class="page-step" id="mount-the-base">Step 2: Mount the base</h2>
<ul>
<li>Place the <a href="models/illumination-base.html">illuminator base</a> onto the <a href="parts/materials/top-plate.html">top plate</a> in opposite orientation to the focusing actuator.</li>
<li>Secure in place with three <a href="parts/mech/M3-10.html">M3x10mm screws</a> and three <a href="parts/mech/nuts.html">M3 nuts</a> (using a <a href="parts/tools/2.5mmBallEndAllenKey.html">2.5mm Ball-end Allen key</a>)</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/mount_base.png" alt="" />
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/mount_base_1.png" alt="" />
<img onmouseover="getElementById('gallery-show0').src=this.src" src="images/mount_base_2.png" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show0" src="images/mount_base.png" alt=""/></div>
<h2 class="page-step" id="assemble-the-slider">Step 3: Assemble the slider</h2>
<h3 id="illuminator-thumbscrew">Illuminator thumbscrew</h3>
<ul>
<li>Push a <a href="parts/mech/nuts.html">M3 nut</a> into the slot in the <a href="models/illumination-thumbscrew.html">illuminator thumbscrew</a>.</li>
<li>Screw a <a href="parts/mech/X-M3-20.html">M3x20mm pozi pan head screw</a> into the <a href="models/illumination-thumbscrew.html">thumbscrew</a>. Use a <a href="parts/tools/screwdriver.html">#1 pozidrive screwdriver</a>.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/thumbscrew.png" alt="" />
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/thumbscrew_1.png" alt="" />
<img onmouseover="getElementById('gallery-show1').src=this.src" src="images/thumbscrew_2.png" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show1" src="images/thumbscrew.png" alt=""/></div>
<h3 id="illuminator-slider">Illuminator slider</h3>
<ul>
<li>Positionate a <a href="parts/mech/Hinsert.html">heat insert</a> in the round hole of the <a href="models/illumination-slider.html">illuminator slider</a></li>
<li>Apply heat to the <a href="parts/mech/Hinsert.html">insert</a> (using a <a href="parts/tools/soldiron.html">soldering iron</a>) and use gentle force to push it into position, as described in the <a href="https://hackaday.com/2019/02/28/threading-3d-printed-parts-how-to-use-heat-set-inserts/">guide to use heat inserts</a>.</li>
<li>Screw the <a href="models/illumination-thumbscrew.html">thumbscrew</a> assembly into the <a href="parts/mech/Hinsert.html">heat insert</a> in the <a href="models/illumination-slider.html">illuminator slider</a>.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/slider-insert.png" alt="" />
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/heat-set_insert.gif" alt="" />
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/slider-insert_1.png" alt="" />
<img onmouseover="getElementById('gallery-show2').src=this.src" src="images/thumbscrew-slider_1.png" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show2" src="images/slider-insert.png" alt=""/></div>
<h2 class="page-step" id="mount-the-light-holder">Step 4: Mount the light holder</h2>
<ul>
<li>Put the <a href="models/illumination-slider.html">illumination slider</a> together with <a href="models/led-arm.html">illumination arm</a>, and align the holes.</li>
<li>Use a <a href="models/arm-pin.html">hinge pin</a> to connect them. Insert it into the rectangular side of the <a href="models/illumination-slider.html">slider</a>.</li>
<li>It should take a little bit of force to push it through the holes. A <a href="parts/tools/pliers.html">needle-nose plier</a> can be useful.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show3').src=this.src" src="images/arm-slider.png" alt="" />
<img onmouseover="getElementById('gallery-show3').src=this.src" src="images/arm-slider_1.png" alt="" />
<img onmouseover="getElementById('gallery-show3').src=this.src" src="images/arm-slider_2.png" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show3" src="images/arm-slider.png" alt=""/></div>
<h2 class="page-step" id="assemble-the-condenser-lens-led-and-heatsink">Step 5: Assemble the condenser lens, LED, and heatsink</h2>
<ul>
<li>Use <a href="parts/consumables/gloves.html">nitrile gloves</a> to manipulate the condenser lens.</li>
<li>Make a V cut in the sides of the <a href="parts/optics/8.7-20mm-circular-lens.html">20 mm circular lens</a> using <a href="parts/tools/precision-wire-cutters.html">precision wire cutter</a> as shown in the images.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show4').src=this.src" src="images/LED3.jpg" alt="" />
<img onmouseover="getElementById('gallery-show4').src=this.src" src="images/LED4.jpg" alt="" />
<img onmouseover="getElementById('gallery-show4').src=this.src" src="images/LED1.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show4" src="images/LED3.jpg" alt=""/></div>
<ul>
<li>Fasten a <a href="parts/elect/high-power-star-LED.html">high-power LED</a> to a <a href="parts/elect/30x30x10mm-heatsink.html">heat sink</a> using a <a href="parts/elect/double-sided-thermal-tape.html">double-sided thermal tape</a>, as described in this <a href="https://youtu.be/ZD3UFZiaZ74">guide</a>.</li>
<li>Fasten the assembly to a <a href="parts/optics/8.7-20mm-circular-lens.html">20 mm circular lens</a> using a <a href="parts/elect/double-sided-adhesive-tape.html">double-sided self adhesive tape</a>, as described in this <a href="https://youtu.be/DwR9CUMfGJU">guide</a>.</li>
<li>The final assembly should be as shown below.</li>
</ul>
<p><img alt="" src="images/LED.jpg" title="" /></p>
<h2 class="page-step" id="assemble-the-illumination-wiring">Step 6: Assemble the illumination wiring</h2>
<ul>
<li>Take the red and black <a href="parts/elect/protoboard-cables.html">cables</a> of the illumination assembly and crimp them using two <a href="parts/elect/male-crimp-pin.html">male connectors</a> and a <a href="parts/tools/crimper.html">crimper</a> as described in this <a href="https://youtu.be/jET1QTP1B7c">guide</a>.</li>
<li>Attach a <a href="parts/elect/2pin-DuPont-female-housing.html">2 pin Du Pont connector female housing</a> to it.</li>
<li>The illumination wiring should be as shown below.</li>
</ul>
<p><img alt="" src="images/2pin-du-pont-connector.png" title="" /></p>
<h2 class="page-step" id="mount-the-illumination-assembly">Step 7: Mount the illumination assembly</h2>
<ul>
<li>Position the illumination assembly in the <a href="models/led-arm.html">illumination arm</a>. One side of the LED holder has a space for cables.</li>
<li>Secure the assembly using a <a href="models/led-cover.html">cover</a>, which also have a space for cables.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show5').src=this.src" src="images/led-assembly-arm.png" alt="" />
<img onmouseover="getElementById('gallery-show5').src=this.src" src="images/led-assembly-arm_1.jpg" alt="" />
<img onmouseover="getElementById('gallery-show5').src=this.src" src="images/led-assembly-arm_2.jpg" alt="" />
<img onmouseover="getElementById('gallery-show5').src=this.src" src="images/led-assembly-arm_3.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show5" src="images/led-assembly-arm.png" alt=""/></div>
<h2 class="page-step" id="attach-the-illumination-wiring">Step 8: Attach the illumination wiring</h2>
<ul>
<li>Insert the illumination wiring to the cable clip next to LED holder</li>
<li>Mount the <a href="models/led-arm.html">illumination arm</a> assembly to the <a href="models/illumination-base.html">base</a>.</li>
<li>Insert the illumination wiring to the other cable clip in the <a href="models/illumination-base.html">base</a>.</li>
</ul>
<div class="gallery-thumb">
<img onmouseover="getElementById('gallery-show6').src=this.src" src="images/cable-clip.jpg" alt="" />
<img onmouseover="getElementById('gallery-show6').src=this.src" src="images/cable-clip_1.jpg" alt="" />
<img onmouseover="getElementById('gallery-show6').src=this.src" src="images/cable-clip_2.jpg" alt="" />
<img onmouseover="getElementById('gallery-show6').src=this.src" src="images/cable-clip_3.jpg" alt="" />
</div>
<div class="gallery-show"><img id="gallery-show6" src="images/cable-clip.jpg" alt=""/></div>
<h2 class="page-step" id="connect-the-strobe">Step 9: Connect the strobe</h2>
<ul>
<li>Connect DuPont female 2-pin connector of the strobe cable to the 2-pin male connector of the illumination wiring. Be careful to connect positive and negative terminals in wrong position.</li>
</ul>
<p><img alt="" src="images/DuPont-2PIN-Connector.jpg" title="" /></p>
<hr />
<p><a href="wiring.html">Previous page</a> | <a href="finished.html">Next page</a></p>
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<p class="author">©2023 Pierre Padilla-Huamantinco, Matías Hurtado-Labarca, and Tobias Wenzel</p>
<p class="email">Contact: <a href="mailto:[email protected]">[email protected]</a></p>
<p class="license">Strobe-enhanced microscopy stage is released under <a href="license.html">CERN-OHL-S-2.0</a>.</p>
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