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cnv_game2fasta.pl
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cnv_game2fasta.pl
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#!/usr/bin/perl -w
#-----------------------------------------------------------+
# |
# cnv_game2fasta.pl - Convert game.xml to fasta file |
# |
#-----------------------------------------------------------+
# |
# AUTHOR: James C. Estill |
# CONTACT: JamesEstill_@_gmail.com |
# STARTED: 12/18/2007 |
# UPDATED: 01/04/2010 |
# |
# DESCRIPTION: |
# Convert sequence data from game.xml file to the fasta |
# file format. Requires XML::Simple |
# |
# USAGE: |
# cnv_game2fata.pl -i infle -o outfile |
# |
# VERSION: $Rev$ |
# |
# |
# LICENSE: |
# GNU General Public License, Version 3 |
# http://www.gnu.org/licenses/gpl.html |
# |
#-----------------------------------------------------------+
package DAWGPAWS;
#-----------------------------+
# INCLUDES |
#-----------------------------+
use strict;
use Text::Wrap; # To print the output wrapped 80 characters
use XML::Simple; # To parse the XML input
use Getopt::Long; # Get options from the command line
# The following needed for printing help
use Pod::Select; # Print subsections of POD documentation
use Pod::Text; # Print POD doc as formatted text file
use IO::Scalar; # For print_help subfunction
use IO::Pipe; # Pipe for STDIN, STDOUT for POD docs
use File::Spec; # Convert a relative path to an abosolute path
#-----------------------------+
# PROGRAM VARIABLES |
#-----------------------------+
my ($VERSION) = q$Rev$ =~ /(\d+)/;
#-----------------------------+
# VARIABLE SCOPE |
#-----------------------------+
my $infile;
my $outfile;
# BOOLEANS
my $quiet = 0;
my $verbose = 0;
my $show_help = 0;
my $show_usage = 0;
my $show_man = 0;
my $show_version = 0;
my $do_test = 0; # Run the program in test mode
# Set the text wrap columns to 60
$Text::Wrap::columns = 60;
#-----------------------------+
# COMMAND LINE OPTIONS |
#-----------------------------+
my $ok = GetOptions(# REQUIRED OPTIONS
"i|infile=s" => \$infile,
"o|outfile=s" => \$outfile,
# ADDITIONAL OPTIONS
"q|quiet" => \$quiet,
"verbose" => \$verbose,
# ADDITIONAL INFORMATION
"usage" => \$show_usage,
"test" => \$do_test,
"version" => \$show_version,
"man" => \$show_man,
"h|help" => \$show_help,);
#-----------------------------+
# SHOW REQUESTED HELP |
#-----------------------------+
if ( ($show_usage) ) {
# print_help ("usage", File::Spec->rel2abs($0) );
print_help ("usage", $0 );
}
if ( ($show_help) || (!$ok) ) {
# print_help ("help", File::Spec->rel2abs($0) );
print_help ("help", $0 );
}
if ($show_man) {
# User perldoc to generate the man documentation.
system ("perldoc $0");
exit($ok ? 0 : 2);
}
if ($show_version) {
print "\ncnv_game2fasta.pl:\n".
"Version: $VERSION\n\n";
exit;
}
#-----------------------------------------------------------+
# MAIN PROGRAM BODY |
#-----------------------------------------------------------+
#-----------------------------+
# FILE IO |
#-----------------------------+
# If not outfile given, append fasta to infile name
unless ($outfile) {
$outfile = $infile.".fasta";
# Will use a hext short name for now
# this only works with the full path is not being used
my $infile_prefix = substr ( $infile , 0, 10 );
$outfile = $infile_prefix.".fasta";
}
open (FASTOUT, ">$outfile") ||
die "Could not open the output file:\n$outfile\n";
# create the xml object
my $game_xml = new XML::Simple;
# read the xml file
my $game_data = $game_xml->XMLin($infile);
#-----------------------------+
# DATA DUMP FOR TESTING |
#-----------------------------+
#use Data::Dumper;
#print STDERR Dumper($game_xml);
#exit;
# lets take a look at what we have here
#use Data::Dumper;
#print STDOUT Dumper($game_data);
#print STDERR "version: ".$game_data->{version}."\n";
my $seq_info = $game_data->{seq};
my $seq_string = $seq_info->{residues};
my $seq_id = $seq_info->{name};
#print STDERR "Seq string".$seq_string."\n";
#print STDERR "seqe".$game_data->{seq->{residues}}."\n";
#print STDERR $game_data->{residues};
# remove blank lines
$seq_string =~ s/\n+/\n/g;
# remove white spaces
$seq_string =~ s/\s+//g;
#-----------------------------+
# PRINT THE FASTA OUTPUT FILE |
#-----------------------------+
# will want to print 80 characters wide
print STDERR $seq_id."\n";
print FASTOUT ">".$seq_id."\n";
#print FASTOUT $seq_string."\n";
print FASTOUT wrap('','', $seq_string);
close (FASTOUT);
exit 0;
#-----------------------------------------------------------+
# SUBFUNCTIONS |
#-----------------------------------------------------------+
sub print_help {
my ($help_msg, $podfile) = @_;
# help_msg is the type of help msg to use (ie. help vs. usage)
print "\n";
#-----------------------------+
# PIPE WITHIN PERL |
#-----------------------------+
# This code made possible by:
# http://www.perlmonks.org/index.pl?node_id=76409
# Tie info developed on:
# http://www.perlmonks.org/index.pl?node=perltie
#
#my $podfile = $0;
my $scalar = '';
tie *STDOUT, 'IO::Scalar', \$scalar;
if ($help_msg =~ "usage") {
podselect({-sections => ["SYNOPSIS|MORE"]}, $0);
}
else {
podselect({-sections => ["SYNOPSIS|ARGUMENTS|OPTIONS|MORE"]}, $0);
}
untie *STDOUT;
# now $scalar contains the pod from $podfile you can see this below
#print $scalar;
my $pipe = IO::Pipe->new()
or die "failed to create pipe: $!";
my ($pid,$fd);
if ( $pid = fork() ) { #parent
open(TMPSTDIN, "<&STDIN")
or die "failed to dup stdin to tmp: $!";
$pipe->reader();
$fd = $pipe->fileno;
open(STDIN, "<&=$fd")
or die "failed to dup \$fd to STDIN: $!";
my $pod_txt = Pod::Text->new (sentence => 0, width => 78);
$pod_txt->parse_from_filehandle;
# END AT WORK HERE
open(STDIN, "<&TMPSTDIN")
or die "failed to restore dup'ed stdin: $!";
}
else { #child
$pipe->writer();
$pipe->print($scalar);
$pipe->close();
exit 0;
}
$pipe->close();
close TMPSTDIN;
print "\n";
exit 0;
}
1;
__END__
=head1 NAME
cnv_game2fasta.pl - Convert game.xml to fasta format file.
=head1 VERSION
This documentation refers to program version $Rev$
=head1 SYNOPSIS
=head2 Usage
cnv_game2fasta.pl -i infile.game.xml -o outfile.fasta
=head2 Required Arguments
--infile # Path to the input file
--outfie # Path to the output file
=head1 DESCRIPTION
This is what the program does
=head1 REQUIRED ARGUMENTS
=over 2
=item -i,--infile
Path of the input file.
=item -o,--outfile
Path of the output file.
=back
=head1 OPTIONS
=over 2
=item --usage
Short overview of how to use program from command line.
=item --help
Show program usage with summary of options.
=item --version
Show program version.
=item --man
Show the full program manual. This uses the perldoc command to print the
POD documentation for the program.
=item -q,--quiet
Run the program with minimal output.
=back
=head1 EXAMPLES
The following are examples of how to use this script
=head2 Typical Use
This is a typcial use case.
=head1 DIAGNOSTICS
=over 2
=item * Expecting input from STDIN
If you see this message, it may indicate that you did not properly specify
the input sequence with -i or --infile flag.
=back
=head1 CONFIGURATION AND ENVIRONMENT
Names and locations of config files
environmental variables
or properties that can be set.
=head1 DEPENDENCIES
Other modules or software that the program is dependent on.
=head1 BUGS AND LIMITATIONS
Any known bugs and limitations will be listed here.
=head1 REFERENCE
A manuscript is being submitted describing the DAWGPAWS program.
Until this manuscript is published, please refer to the DAWGPAWS
SourceForge website when describing your use of this program:
JC Estill and JL Bennetzen. 2009.
The DAWGPAWS Pipeline for the Annotation of Genes and Transposable
Elements in Plant Genomes.
http://dawgpaws.sourceforge.net/
=head1 LICENSE
GNU General Public License, Version 3
L<http://www.gnu.org/licenses/gpl.html>
=head1 AUTHOR
James C. Estill E<lt>JamesEstill at gmail.comE<gt>
=head1 HISTORY
STARTED:
UPDATED:
VERSION: $Rev$
=cut
#-----------------------------------------------------------+
# HISTORY |
#-----------------------------------------------------------+
#
# 01/04/2010
# - Using xml::simple, only about 30% of the game.xml files
# are being properly parsed. Working on alternative methods.